Native soft tissue matrix for therapeutic applications
Inventors
Awad, Hani • Estes, Bradley T. • Guilak, Farshid
Assignees
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Publication Number
US-11357889-B2
Publication Date
2022-06-14
Expiration Date
Abstract
A method is used for preparing a product for use in repairing a lesion or defect at a tissue site in a human or animal patient body. The method includes obtaining tissue from a donor human or animal body and freezing the obtained tissue. The method further includes pulverizing the frozen tissue and suspending the pulverized tissue in a fluid. The method further includes homogenizing the tissue suspension and precipitating tissue particles from the homogenized tissue suspension. The method further includes re-suspending the precipitated tissue particles and lyophilizing the tissue re-suspension to provide the product to be used in repairing the lesion or defect.
Core Innovation
The invention provides a method for preparing a product for use in repairing a lesion or defect at a tissue site in a human or animal patient body. The method includes obtaining tissue from a donor human or animal body, freezing the obtained tissue, pulverizing the frozen tissue, suspending the pulverized tissue in a fluid, homogenizing the tissue suspension using a tissue homogenizer, precipitating tissue particles from the homogenized tissue suspension, re-suspending the precipitated tissue particles, and lyophilizing the tissue re-suspension to provide the product.
The prepared product is used for repairing a lesion or defect by reconstituting a dry formulation to form a slurry and implanting the slurry into the lesion or defect. In this repairing method, tissue is obtained from a donor human or animal body, frozen, pulverized, suspended in a fluid, homogenized, precipitating tissue particles, re-suspending the precipitated tissue particles, lyophilizing to form a dry formulation, reconstituting the dry formulation to form a slurry, and implanting the slurry into the lesion or defect at the tissue site.
The described implementations further encompass formats in which the slurry is used to form or associate with implantable tissue repair structures. The slurry can be converted into a porous solid scaffold for implantation into the lesion or defect, or bound to a scaffold such that the scaffold is implanted into the lesion or defect.
Claims Coverage
The document contains two independent claims, covering a preparation method that ends with a lyophilized product from precipitated tissue particles and a repair method that extends preparation by reconstituting to a slurry and implanting into a lesion or defect. Across the claim sets, the core inventive features are organized around donor tissue processing followed by lyophilization, then reconstitution and implantation, including scaffold-related variants.
Donor tissue processing to lyophilized product
Obtaining tissue from a donor human or animal body, freezing the obtained tissue, pulverizing the frozen tissue, suspending the pulverized tissue in a fluid, homogenizing the tissue suspension using a tissue homogenizer, precipitating tissue particles from the homogenized tissue suspension, re-suspending the precipitated tissue particles, and lyophilizing the tissue re-suspension to provide the product to be used in repairing the lesion or defect.
Reconstituting dry formulation to slurry and implanting for repair
Obtaining tissue from a donor human or animal body, freezing the obtained tissue, pulverizing the frozen tissue, suspending the pulverized tissue in a fluid, homogenizing the tissue suspension using a tissue homogenizer, precipitating tissue particles from the homogenized tissue suspension, re-suspending the precipitated tissue particles, lyophilizing the tissue re-suspension to form a dry formulation, reconstituting the dry formulation to form a slurry, and implanting the slurry into the lesion or defect at the tissue site.
Porous solid scaffold implantation using the slurry
Forming a porous solid scaffold from the slurry and implanting the porous solid scaffold into the lesion or defect.
Slurry bound to a scaffold for implantation
Binding the slurry to a scaffold and implanting the scaffold into the lesion or defect.
The claim coverage focuses on a tissue-derived processing workflow that produces a lyophilized product or dry formulation from donor tissue through freezing, pulverizing, suspension, homogenization, precipitation, re-suspension, and lyophilization. Repair is accomplished by reconstituting to a slurry and implanting into a lesion or defect, with scaffold-related variants.
Stated Advantages
Documented Applications
No documented applications found
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