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Publication Number
US-11345886-B2
Publication Date
2022-05-31
Expiration Date
Abstract
The presently disclosed subject matter relates, in general, to the identification, isolation, and use of a population of stem cells isolated from umbilical cord blood, peripheral blood and/or other sources and that are referred to herein as Small Mobile Stem cells (short: SMS). More particularly, the presently disclosed subject matter relates to isolating said SMS stem cells and employing the same, optionally after in vitro manipulation, to treat tissue and/or organ damage in a subject in need thereof.
Core Innovation
The disclosed subject matter relates to Small Mobile Stem (SMS) cells and to producing and identifying a tubule from extracellular matrix (ECM) protein derived from small mobile stem (SMS) cells. SMS cells are isolated from umbilical cord blood, peripheral blood, bone marrow, and various solid tissues, and are characterized as very small and highly mobile. SMS cells show strong plastic adherence and long-term proliferation, are resistant to adverse conditions, and can be grown in serum-free conditions while producing extracellular matrix (ECM) and organized ECM layers.
The disclosure further characterizes SMS-derived differentiation into multiple lineages, including osteogenic, adipogenic, and neurogenic differentiation. Neurogenic differentiation is associated with expression of neuronal markers, including class III beta tubulin, GFAP, and PLP. In vitro, SMS cells form complex multicellular macrostructures associated with tubulogenesis-related architectures, including parallel duplicate membrane incisions, three-dimensional cluster mesh assemblies, coated tubular assemblies, two-dimensional leaf mesh assemblies, crescent pre-tubular structures, membrane-to-tube transitions, and fenestrations.
The document describes producing tubules from ECM obtained from a population of isolated SMS cells by culturing the ECM under conditions that promote tubule formation, and identifying a tubule produced in the culture. The growth media is described as comprising Dulbecco’s Modified Eagle’s medium (DMEM) and heat inactivated fetal bovine serum. The tubule-associated structures can be produced and identified as tubules in culture, including specific structural forms such as cluster mesh assemblies, coated tubular assemblies, and leaf mesh assemblies.
Claims Coverage
The document provides one independent claim directed to a method of producing and identifying a tubule from ECM protein from small mobile stem (SMS) cells, with inventive features centering on culturing SMS-cell-derived ECM under tubule-promoting conditions and using a growth media comprising DMEM and heat inactivated fetal bovine serum.
Culturing SMS-derived ECM under tubule-promoting conditions
Culturing ECM obtained from a population of isolated SMS cells under conditions, which promote the formation of a tubule.
Identifying a tubule produced in the culture
Identifying a tubule produced in the culture.
Using DMEM and heat inactivated fetal bovine serum in growth media
Culturing the ECM in a growth media comprising a Dulbecco’s Modified Eagle’s medium and heat inactivated fetal bovine serum.
Overall, the independent claim focuses on producing and identifying a tubule by culturing ECM obtained from isolated SMS cells in growth media comprising DMEM and heat inactivated fetal bovine serum under conditions that promote tubule formation.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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