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Publication Number
US-11339300-B2
Publication Date
2022-05-24
Expiration Date
Abstract
Disclosed herein are methods for preventing biofilm formation on a surface. The present disclosure also relates to anti-biofilm forming agents, to methods of producing and using them, and to anti-fouling coatings produced therefrom.
Core Innovation
The disclosure describes reef-fish mucosal microflora as sources of antagonistic bacteria. These antagonistic bacteria inhibit biofilm formation and eukaryotic fouling, and the inhibition is associated with stationary-phase secondary metabolites and/or living cells derived from the reef-fish mucosal microflora.
The problem addressed is biofilm formation and biofouling, including the need for resistance and eco-friendly anti-fouling agents. The disclosure frames antagonistic reef-fish skin microflora as a means to inhibit bacterial biofilm formation and also inhibit eukaryotic fouling, including both photosynthesizing and non-photosynthesizing eukaryotic cells.
The disclosure supports the concept by isolating and characterizing multiple fish skin bacterial isolates, growing them to a stationary phase, and evaluating antibacterial activity and anti-biofilm inhibition. The identified active isolates and their corresponding extracts are further characterized by fatty-acid profiling and 16S rRNA with GC and DNA-DNA hybridization, including strain relationships to genera and species such as Staphylococcus warneri and Aerococcus viridans and candidate new taxa.
Claims Coverage
The independent claim clm-00001 is directed to a culture medium composition for growing one or more specified bacteria identified by strain designations, with the inventive coverage centered on the defined culture medium components. Dependent claims further refine the medium composition and also constrain the bacterial growth state, including stationary phase and a stated growth duration, and provide downstream compositions and uses based on culture by-products and extracts, including anti-fouling and anti-biofilm functions.
Culture medium for growing specified bacteria strains
A composition comprising a culture medium for growing one or more bacteria identified as PTA-6763, PTA-6682, PTA-6764, PTA-6681, PTA-6765, or PTA-6766, wherein the culture medium comprises one or more bacteria identified as PTA-6763, PTA-6682, PTA-6764, and PTA-6681, and wherein the medium comprises one or more of agar, artificial sea water (ASW) medium, tryptic soy broth (TSB), brain heart infusion (BHI) broth, nutrient broth, and marine broth.
Specified agar component(s) in the culture medium
The culture medium comprises one or more of the listed agar types.
Growing bacteria to stationary phase
The culture medium includes one or more bacteria grown to stationary phase.
Growing bacteria for a specified duration
The culture medium includes one or more bacteria grown for 48 hours.
Culture supernatant as a composition
The composition includes supernatant from a culture medium used to grow bacteria identified as PTA-6763, PTA-6682, PTA-6764, PTA-6681, PTA-6765, or PTA-6766.
Contacting a surface to inhibit biofilm or microbial effects
A method that inhibits biofilm formation on a surface, inhibits microorganism growth, or kills microorganisms by contacting the surface with the composition of a downstream claim.
Overall, the claims focus on growing defined antagonistic bacterial isolates in a culture medium defined by selected agar and broth components, optionally under constrained growth conditions, and then using culture-derived products such as supernatant and extracts in contact-based applications directed to inhibiting biofilm formation and eukaryotic and microbial fouling effects.
Stated Advantages
Inhibits biofilm formation.
Inhibits microorganism growth and can kill microorganisms.
Inhibits eukaryotic fouling, including photosynthesizing and non-photosynthesizing eukaryotic cells.
Enables eco-friendly anti-fouling agents and coatings based on antagonistic reef-fish mucosal microflora.
Documented Applications
Anti-fouling marine paints and coatings using the described bacterial isolates and/or their culture-derived materials.
Preventing fouling or inhibiting biofilm and microbial growth upon contacting surfaces.
Anti-biofilm uses described in connection with medical and industrial contexts.
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