Biofilm inhibiting compositions enhancing weight gain in livestock
Inventors
Mahdavi, Jafar • Ala'Aldeen, Dlawer • Soultanas, Panos
Assignees
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Publication Number
US-11311511-B2
Publication Date
2022-04-26
Expiration Date
Abstract
A method of enhancing the growth of an animal, as well as treating or preventing antimicrobial infections is provided. The method includes causing the animal to ingest or absorb an effective amount of one or more Fe III complex compounds, including but not limited to Fe III complexes comprising ligands bound to the iron center such as amino acids or α-hydroxy acids. The compounds are also useful for inhibiting, reducing, or preventing biofilm formation or buildup on a surface; the treatment of, inhibition of growth of, and inhibition of colonization by, bacteria, both in biological and non-biological environments; disinfecting surfaces, potentiating the effects of antibiotics and other anti-microbial agents, and increasing the sensitivity of bacteria and other microorganisms, to anti-microbial agents are also provided.
Core Innovation
The invention provides a milk replacer composition comprising one or more Fe(III) complex compounds of Formula I. The Fe(III) complexes include ligands that are the conjugate base of a specified α-hydroxy acid or the conjugate base of a specified amino acid, and salts and/or hydrates of these complexes are included.
At least one of the one or more Fe(III) complexes is present in a concentration ranging from 0.01 g/Kg to 20 g/Kg of the milk replacer composition. The Formula I definition sets x as 1-2 and y as 1-3, and the disclosed named compounds include ferric lactate, ferric citrate, and ferric tartrate.
The described content further states that the Fe(III) complex family inhibits bacterial attachment and/or biofilm formation, inhibits binding of Campylobacter MOMPs/FlaA, potentiates antibiotics, and resensitizes resistant strains. The same compounds are described in animal feed contexts for improved growth performance and feed efficiency while reducing biofilm or colonization.
Claims Coverage
The claim coverage centers on one independent milk replacer composition claim with one core inventive configuration and additional dependent refinements. The inventive features are the Formula I Fe(III) complexes and the specified concentration range.
Formula I Fe(III) complexes in a milk replacer composition
A milk replacer composition comprising one or more Fe(III) complex compounds of Formula I, wherein x is 1-2 and y is 1-3, and each ligand is independently a conjugate base of a specified α-hydroxy acid or a specified amino acid, including salts and/or hydrates.
Specified Fe(III) complex concentration in milk replacer
At least one of the one or more Fe(III) complexes is present in a concentration ranging from 0.01 g/Kg to 20 g/Kg of the milk replacer composition.
The claim set covers milk replacer compositions containing Fe(III) complexes of Formula I with ligands selected from specified α-hydroxy acids or amino acids, including salts and/or hydrates, and at least one complex present at 0.01 g/Kg to 20 g/Kg. Dependent claims further narrow concentration sub-ranges and specify particular Formula I compounds and growth-related criteria.
Stated Advantages
Reduces enteric and pulmonary bacterial burden in described animal studies.
Improves zootechnical outcomes, including improved feed conversion and growth-related performance in the described studies.
Inhibits biofilm formation in described experiments with clinical CF lung isolate strains.
Promotes growth in the described chicken study outcomes and shows reductions in Campylobacter, Salmonella, and E. coli CFU counts.
Reduces feed conversion ratio and improves calf body weight in the described calf milk replacer trial.
Potentiating antibiotics and resensitizing resistant strains.
Reduction or inhibition of biofilm formation, bacterial attachment, or colonization.
Enhancing mammalian growth as determined by measurable growth-related characteristics.
Improved feed efficiency.
Documented Applications
Weaned piglets are treated with feed including water-soluble Fe(III) complexes, with reported reductions in enteric/pulmonary bacterial burden and improved feed conversion outcomes in the described study.
Biofilm inhibition is evaluated for clinical CF lung isolate strains using ferric EDTA, ferric malate, and ferric oxalate hydroxylate in described bead assays with CFU quantitation.
Chickens (Ross 308 male broilers) are reared with feed containing FeTyr to address Campylobacter burden in caecal contents and to support growth promotion outcomes, with reported reductions in Campylobacter, Salmonella, and E. coli CFU counts.
Rearing calves receive calf milk replacer supplemented with ferric citrate (CI-PLEX Chelate) in addition to normal-rate ferrous sulfate, with reported improved body weight and reduced feed conversion ratio over a 56-day trial and some improvements in health-related clinical scores.
A milk replacer composition for livestock use.
Animal feed applications for pigs and poultry.
Biofilm reduction or disinfection in household, medical, and cosmetic contexts.
Treatment of microbial infections associated with biofilm formation.
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