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Publication Number
US-11280711-B2
Publication Date
2022-03-22
Expiration Date
Abstract
A method including placing a biological sample taken from a body into a chamber; adding a composition including an acetal solvent to the chamber; and fixating the biological sample. A method including placing a tissue from a body into a chamber; and contacting the tissue with a composition including an acetal solvent as a fixating process.
Core Innovation
The described subject matter provides tissue-processing reagents and methods in which an acetal solvent is used to fixate and perform histology pre-analytical processing steps. The acetal solvent is preferably 1,3-dioxolane, with related acetals and adducts also described, including methylal, ethylal, butylal, dioxolane/glycerol formal, acetaldehyde diethyl acetal, and adducts.
The disclosure explains that 1,3-dioxolane can function alone or be mixed with dehydrants and clearing agents, including alcohols, acetone, xylene, and glycols. It also describes dioxolane as an additive in paraffin, with the quantity described as up to about 20%.
The disclosure further describes in-situ hydrolysis of dioxolane to generate formaldehyde for fixation while residual dioxolane remains present to support the processing. A catalyst and water mixture is described for hydrolysis, including acid or Lewis acid catalysts such as hydrochloric acid and other specified catalysts.
Claims Coverage
The partial content includes one independent claim. The independent claim contains one inventive feature centered on a specific composition formulation and its use context for processing a biological sample prior to paraffin introduction, with a quantitative limitation for 1,3-dioxolane content.
Paraffin pre-treatment composition with limited 1,3-dioxolane content
A composition for use in a treatment of a biological sample taken from a body prior to containing the biological sample with a paraffin, comprising 1,3-dioxolane and a second agent of a paraffin wax, where the 1,3-dioxolane is present in an amount of 20 percent by volume or less.
The independent claim requires a pre-paraffin treatment composition that includes 1,3-dioxolane and a paraffin wax second agent, while limiting 1,3-dioxolane to 20 percent by volume or less, for treatment of a biological sample taken from a body prior to containing the sample with a paraffin.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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