Compositions comprising recombinant bacillus cells and an insecticide
Inventors
CURTIS, Damian • Thompson, Brian
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-11266150-B2
Publication Date
2022-03-08
Expiration Date
Abstract
The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.
Core Innovation
The invention provides a crop-protection composition that uses recombinant exosporium-producing Bacillus cells derived from Bacillus thuringiensis BT013A, deposited under Agricultural Research Service Culture Collection Accession NRRL No. 50924. The recombinant Bacillus cells express a fusion protein comprising an endoglucanase and an exosporium targeting sequence, an exosporium protein, or an exosporium protein fragment comprising an exosporium targeting sequence.
The endoglucanase comprises an amino acid sequence with at least 95% sequence identity to SEQ ID NO: 107, and the exosporium targeting component is configured to target exosporium using the specified targeting sequence, exosporium protein, or fragment. The targeting component includes variants and fragments of exosporium proteins such as BclA, BclB, BetA/BAS3290, and related exosporium proteins described in the document.
The composition also includes at least one insecticide selected from a specified group in a synergistically effective amount. The disclosed use includes treating a plant, a plant part, or the locus surrounding the plant by simultaneously or sequentially applying the recombinant exosporium-producing Bacillus cells together with the insecticide, and the document also describes seed treatment use, including coating a seed with the claimed composition.
Claims Coverage
The document includes two independent claims covering a composition and a method. Across these independent claims, the core inventive features combine recombinant exosporium-producing Bacillus cells expressing a defined endoglucanase-containing fusion protein with an exosporium targeting component, and a specified insecticide or an insecticide in a synergistically effective amount used in a plant treatment method.
Recombinant exosporium-producing Bacillus fusion protein with defined endoglucanase and exosporium targeting component
Recombinant exosporium-producing Bacillus cells derived from Bacillus thuringiensis BT013A (NRRL No. 50924) that express a fusion protein comprising an endoglucanase with an amino acid sequence with at least 95% sequence identity to SEQ ID NO: 107, and an exosporium targeting sequence, an exosporium protein, or an exosporium protein fragment comprising an exosporium targeting sequence.
Synergistic combination with an enumerated insecticide
A synergistically effective amount including at least one insecticide selected from the group consisting of acetamiprid, aldicarb, amitraz, beta-cyfluthrin, carbaryl, clothianidin, cyfluthrin, cypermethrin, deltamethrin, endosulfan, ethion, ethiprole, ethoprophos, fenamiphos, fenobucarb, fenthion, fipronil, flubendiamide, fluopyram, flupyradifurone, formetanate, heptanophos, imidacloprid, methamidophos, methiocarb, methomyl, niclosamide, oxydemeton-methyl, phosalone, silafluofen, spirodiclofen, spiromesifen, spirotetramat, thiacloprid, thiodicarb, tralomethrin, triazophos, triflumuron, vamidothion, 1-{2-fluoro-4-methyl-5-[(R)-(2,2,2-trifluoroethyl)sulphinyl]phenyl}-3-(trifluoromethyl)-1H-1,2,4-triazol-5-amine, and 1-(3-chloropyridin-2-yl)-N-[4-cyano-2-methyl-6-(methylcarbamoyl)phenyl]-3-{[5-(trifluoromethyl)-2H-tetrazol-2-yl]methyl}-1H-pyrazole-5-carboxamide.
Plant treatment by simultaneous or sequential application to enhance growth and/or plant health
A method of treating a plant, a plant part, or the locus surrounding the plant to enhance plant growth and/or promote plant health comprising the step of simultaneously or sequentially applying recombinant exosporium-producing Bacillus cells expressing a fusion protein comprising an endoglucanase with at least 95% sequence identity to SEQ ID NO: 107 and an exosporium targeting sequence, exosporium protein, or fragment together with at least one insecticide in a synergistically effective amount; wherein the recombinant Bacillus cells are derived from Bacillus thuringiensis BT013A (NRRL No. 50924).
Across independent claims clm-00001 and clm-00010, the claims are directed to recombinant exosporium-producing Bacillus cells derived from Bacillus thuringiensis BT013A that express an endoglucanase-containing fusion protein linked to an exosporium targeting component, combined with an insecticide in a synergistically effective amount for a composition and for treating a plant to enhance growth and/or promote plant health. Dependent claims further constrain the exosporium targeting component using specified SEQ ID NO: 1 region identity thresholds and SEQ ID NO: 2 identity thresholds, and optionally restrict the insecticide to specific named compounds.
Stated Advantages
Enhance plant growth and/or promote plant health.
Control insects, mites, nematodes, and phytopathogens as described in the document summary context.
Improve germination and emergence when used as a seed treatment as described in the document summary context.
Synergistically effective amount and synergism is evaluated with Colby equation and Tammes isoboles method, with reporting of superadditive plant growth/yield effects in the partial content.
Documented Applications
Treating a plant, a plant part, or the locus surrounding the plant by simultaneously or sequentially applying the recombinant exosporium-producing Bacillus cells with the insecticide in a synergistically effective amount to enhance plant growth and/or promote plant health.
Seed treatment by coating a seed with the claimed composition.
Seed-dressing formulations are described for application to crop, ornamental, and GM plants, including use on transgenic seeds.
Combination use is documented in the partial content for superadditive plant growth/yield effects from combining flupyradifurone with a recombinant Bacillus thuringiensis BT013A exosporium-associated phospholipase C product (BEPC).
Interested in licensing this patent?