Polymeric surface having reduced biomolecule adhesion to thermoplastic articles of such substrate
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Publication Number
US-11248100-B2
Publication Date
2022-02-15
Expiration Date
Abstract
A substrate is described having a treated contact surface comprising a carbon or silicon compound comprising from 1 to 30 atomic percent oxygen, from 0.1 to 30 atomic percent nitrogen, or both, each as measured by XPS. The treated contact surface has a biomolecule recovery percentage greater than the biomolecule recovery percentage of the surface before treatment according to the method.
Core Innovation
The invention relates to thermoplastic articles, including microplates, having a fluid contact surface and an interior portion comprising polypropylene or polystyrene. The fluid contact surface is defined by X-ray photoelectron spectroscopy (XPS) atomic percentages of grafted oxygen atoms, optionally grafted nitrogen atoms, and carbon atoms in specified ranges, and the formed microplate provides a biomolecule recovery percentage of at least 85% after 24 hours.
The invention further provides a treatment sequence for forming the fluid contact surface, comprising treating the fluid contact surface with a conditioning plasma comprising oxygen followed by a conversion plasma comprising water vapor. The conversion plasma is generated at a remote point from the fluid contact surface, and the process includes a constraint on plasma radiative energy distribution expressed as a ratio of radiant energy density at the fluid contact surface to radiant energy density at a brightest point of the conversion plasma that is less than 0.5.
The disclosure also addresses improved biomolecule recovery through plasma-conditioned contact surfaces, with claims of minimal extractables/inorganics and grafting without coating. Diverse biomolecules are referenced, including BSA, fibrinogen, transferrin, and Protein A/Protein G, with microplates and ELISA plates referenced as vessel formats.
Claims Coverage
The document includes independent claim coverage defining the microplate structure and the plasma-based treatment conditions. The inventive features combine XPS-defined surface composition, a polypropylene or polystyrene interior, oxygen conditioning followed by remote water-vapor conversion plasma, a radiant-energy-density ratio constraint, and biomolecule recovery performance after 24 hours.
XPS-defined fluid contact surface on polypropylene or polystyrene microplate
A microplate having a fluid contact surface and an interior portion comprising polypropylene or polystyrene, where the fluid contact surface comprises grafted oxygen atoms, optional grafted nitrogen atoms, and carbon atoms with atomic % values measured by XPS, and where the fluid contact surface has a biomolecule recovery percentage of at least 85% after 24 hours.
Oxygen conditioning plasma followed by remote water-vapor conversion plasma
A method including providing a microplate and treating the fluid contact surface with a conditioning plasma comprising oxygen followed by a conversion plasma comprising water vapor, where the conversion plasma is generated at a remote point from the fluid contact surface.
Radiant energy density ratio constraint for remote conversion plasma
The method where a ratio of radiant energy density at the fluid contact surface to radiant energy density at a brightest point of the conversion plasma is less than 0.5.
The independent claim coverage centers on forming an XPS-defined fluid contact surface on a polypropylene or polystyrene microplate to achieve high biomolecule recovery, by performing oxygen conditioning plasma followed by a remotely generated water-vapor conversion plasma under a radiant energy density ratio constraint.
Stated Advantages
Improved biomolecule recovery, with a biomolecule recovery percentage of at least 85% after 24 hours.
Reduced non-specific protein/biomolecule adhesion as stated for covalently bound zwitterionic monolayer coatings.
Improved biomolecule recovery as stated for covalently bound zwitterionic monolayer coatings.
Claims of minimal extractables/inorganics and grafting without coating are supported in the disclosed content.
Documented Applications
Use of the treated microplate/ELISA plate formats for biomolecule recovery, referenced with biomolecules including BSA, fibrinogen, transferrin, and Protein A/Protein G.
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