Soluble balls for preparing solutions
Inventors
Fortin, Tanguy • BARDET, Chloe • BIARC, Jordane
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-11237084-B2
Publication Date
2022-02-01
Expiration Date
Abstract
The present invention concerns the preparation of solutions, particularly for implementing analytical methods, in particular by spectrometry, particularly for producing standard solutions which are useful, for example, for calibrating spectrometers or for implementing diagnostic methods. It allows the implementation of an easy process for preparing such standard solutions.
Core Innovation
The invention relates to soluble, calibrated beads carrying grafted products, where the beads are dissolved to prepare standard aqueous or hydroalcoholic solutions for spectrometric and diagnostic uses. The process uses one or more soluble beads having a surface, in which at least ten different products to be dissolved are present at the surface and grafted thereon. The beads are calibrated with a homogeneous diameter ranging from 1 to 4 mm, and each bead carries a predetermined amount of each of the at least ten different products grafted onto its substantially equal surface.
The grafted products are defined as peptides, proteins and small molecules, and can be isotopically labelled and/or deuterated and/or contain post-translational modifications such as phosphorylation, oxidation and carbamidomethylation. The prepared solution is made by dissolving the calibrated soluble beads, with the amount of products grafted onto each bead predetermined from 1 picogram to 100 micrograms per bead. The bead material is water-soluble and can include sucrose, lactose, alginates and lactic acid, including mixtures of water-soluble compounds such as sugars, polysaccharides and hydroxycarboxylic acids.
The invention further addresses calibration standard preparation by enabling dissolution and release of grafted products without interference with spectrometers. It relies on grafting as reversible adsorption enabling complete release after dissolution, while bead sets can be made with controlled release packaging and ranges of calibration standards by varying graft load between sets. Documented examples include peptide-grafted sucrose beads used for LC-UV and LC-MS, preparation of peptides and quantified analytes such as apolipoprotein E in plasma using a peptide-grafted bead, and preservation of enzyme activity such as trypsin after grafting, supporting preparation of predetermined protein/enzyme solutions without denaturation.
Claims Coverage
The provided excerpt includes three independent claims that collectively cover (i) a process for preparing an aqueous solution by dissolving calibrated soluble beads, (ii) a set of soluble beads carrying predetermined amounts of at least ten different grafted products, and (iii) a process for preparing such soluble beads by impregnating polymer beads, grafting products onto the bead surfaces, and drying, with calibrated beads and predetermined grafted product amounts.
Calibrated soluble bead dissolution for aqueous solution preparation
A process for preparing an aqueous solution of at least ten different products to be dissolved by dissolving in the solvent of the aqueous solution one or more soluble beads having a surface, where the at least ten products to be dissolved are present at the surface and grafted thereon, and where the beads are calibrated with a homogeneous diameter ranging from 1 to 4 mm.
Set of soluble beads with substantially equal surface loading
A set of soluble beads carrying at least ten different products to be analysed, grafted onto the surface of each soluble bead, wherein the beads each have a predetermined amount of the at least ten different products grafted onto their substantially equal surface.
Impregnation, grafting, and drying to produce calibrated beads with predetermined loading
A process for preparing soluble beads carrying at least ten different products to be analysed grafted onto the surface of each soluble bead, comprising a first step of impregnating polymer beads with a solution containing the product(s) to allow grafting, then a step of drying the grafted beads obtained, where the beads are calibrated with a homogeneous diameter and the amount of product grafted onto each bead is predetermined from 1 picogram to 100 micrograms per bead.
Across the independent claims, the core inventive concept is based on using calibrated soluble beads (1 to 4 mm homogeneous diameter) whose surfaces carry at least ten different grafted products with predetermined loading (1 picogram to 100 micrograms per bead). The claims cover both preparing the bead set (including impregnating polymer beads, grafting, and drying) and preparing aqueous solutions (and standards) by dissolving the calibrated soluble beads, with an intended use for analysis in spectrometric analysis methods as reflected in the dependent claim refinements provided.
Stated Advantages
Avoidance of interference with spectrometers during spectrometric analysis.
Grafting is reversible adsorption enabling complete release after dissolution.
Preparation of predetermined product solutions by dissolving beads with controlled graft load and standard ranges for calibration.
Preservation of enzyme activity after grafting, supporting preparation of predetermined protein/enzyme solutions without denaturation.
Documented Applications
Preparation of standard aqueous/hydroalcoholic solutions for spectrometric analysis and diagnostic methods by dissolving peptide/protein/small-molecule grafted beads.
Preparation and use of peptide-grafted beads for LC-UV and LC-MS analysis.
Quantification of apolipoprotein E in plasma using a peptide-grafted bead.
Use of calibrated bead solutions as calibration standards for spectrometers, including quality control of spectrometers.
Interested in licensing this patent?