Apparatus for automatic sampling of biological species employing disk microfluidics system
Inventors
Shachar, Josh Yehoshua • Gurley, Sam • CIPRIANO, Aaron • Yin, Peter • Purnell, Rob • Rocklinger, Marc • Petrullo, Ming • Stelea, Stelica
Assignees
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Publication Number
US-11224874-B2
Publication Date
2022-01-18
Expiration Date
Abstract
A field portable diagnostic apparatus uses a rotatable disk in which a microfluidic circuit is defined. The microfluidic circuit includes a centrifugal separation chamber receiving a sample to stratify the sample. A magnetic bead holding chamber is communicated to a mixing chamber, where mass amplifying functionalized magnetic-nanoparticles, held in a buffer solution and contained in the magnetic bead holding reservoir communicated to mixing chamber, are mixed with the separated fluid delivered to mixing chamber from the separation chamber. The functionalized magnetic nanoparticles conjugate with a target analyte in the sample. A magnet in proximity to a SAW chamber including a SAW detector draws the functionalized magnetic nanoparticles toward antibodies immobilized on the SAW sensor surface A wash reservoir is communicated to the SAW sensor chamber, and a cleanup/waste reservoir is communicated to the SAW chamber for receive fluid after it has passed through the SAW chamber.
Core Innovation
The invention provides a field portable diagnostic apparatus that uses a rotatable disk microfluidic circuit. A septum enables insertion of a sample with a target analyte into a sample reservoir sealed by the septum, and the disk includes a separation chamber communicated to the sample reservoir, where centrifugal forces stratify the sample into separated fluid delivered downstream.
The disk also includes a magnetic bead holding reservoir holding mass amplifying functionalized magnetic-nanoparticles in a buffer solution. The functionalized magnetic-nanoparticles are mixed in a mixing chamber with the separated fluid, where the functionalized magnetic-nanoparticles conjugate with the target analyte in the mixing chamber.
Conjugated magnetic-nanoparticles are then delivered to a surface acoustic wave detector having a SAW chamber and a SAW sensor surface. A movable magnet is selectively positionable in proximity to the SAW chamber to draw the functionalized magnetic-nanoparticles toward antibodies immobilized on the SAW sensor surface, and a PBS wash followed by DI water wash is released sequentially through valve controlled channels, with waste held after it passes through the SAW chamber.
Claims Coverage
The independent claim contains multiple inventive features distributed across the rotatable disk, centrifugal stratification, magnetic nanoparticle mass amplification and conjugation, movable-magnet nanoparticle drawing to immobilized antibodies on a SAW sensor surface, and valve-controlled PBS/DI washing and waste routing.
Field portable diagnostic apparatus with a rotatable disk microfluidic circuit
A field portable diagnostic apparatus comprising a rotatable disk having a center and a septum for providing closure of a microfluidic circuit while allowing insertion of a sample with a target analyte.
Centrifugal sample separation chamber stratifying the sample
A sample reservoir sealed by the septum for holding the sample and a separation chamber communicated to the sample reservoir, where centrifugal forces stratify the sample.
Mass amplifying functionalized magnetic-nanoparticles conjugating in a mixing chamber
A magnetic bead holding reservoir communicated to a mixing chamber through a separation siphon, where mass amplifying functionalized magnetic-nanoparticles held in a buffer solution are mixed with separated fluid delivered to the mixing chamber, conjugating with a target analyte in the sample.
SAW sensing chamber with movable magnet drawing nanoparticles to immobilized antibodies
A surface acoustic wave detector having a SAW sensor surface and a SAW chamber communicated to the mixing chamber through a first valve controlled channel, where a movable magnet is selectively positionable in proximity to the SAW chamber to draw the functionalized magnetic-nanoparticles toward antibodies immobilized on the SAW sensor surface.
Sequential PBS wash and DI water wash with waste siphon and cleanup/waste reservoir
A phosphate buffered saline wash reservoir communicated to the SAW sensor chamber through a second valve controlled channel and a deionized water wash reservoir communicated with the SAW sensor chamber through a third valve controlled channel with sequential release, together with a waste siphon communicated to the SAW chamber and a cleanup/waste reservoir communicated by the waste siphon for holding fluid after it has passed through the SAW chamber.
The inventive coverage combines a rotatable disk for centrifugal stratification and valve-controlled fluid routing, magnetic bead-based mixing with mass amplifying functionalized magnetic-nanoparticles that conjugate with a target analyte, and SAW detection using a movable magnet to draw conjugated nanoparticles toward antibodies immobilized on the SAW sensor surface, including sequential PBS and DI water washing and downstream waste handling.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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