Antimicrobial compositions
Inventors
Patton, Thomas • Brennan, James • Barrett, John Reginald • STAPLES, Ian
Assignees
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Publication Number
US-11185080-B2
Publication Date
2021-11-30
Expiration Date
Abstract
Storage-stable compositions for generating antimicrobial activity are described. The compositions comprise an enzyme that is able to convert a substrate to release hydrogen peroxide, and an unrefined natural substance, such as a honey, that includes a substrate for the enzyme. In certain embodiments, the enzyme is a purified enzyme. In other embodiments, the substrate lacks catalase activity, and the enzyme is additional to any enzyme activity able to convert the substrate to release hydrogen peroxide that may be present in the unrefined natural substance. The storage-stable compositions do not include sufficient free water to allow the enzyme to convert the substrate. Use of the compositions to treat microbial infections and wounds is described, as well as methods for their production.
Core Innovation
The invention relates to a sterile storage-stable composition for generating antimicrobial activity for treating microbial infections. The composition includes an enzyme able to convert a substrate to release hydrogen peroxide, and a honey that includes a substrate for the enzyme, while the honey lacks catalase activity and lacks glucose oxidase activity.
The composition is configured to be substantially free of hydrogen peroxide prior to use, and it does not include sufficient free water to allow the enzyme to convert the substrate, or it has a water activity of 0.47 to 0.7. This limited free water or specified water activity prevents premature hydrogen peroxide generation during storage and supports sterility and storage stability.
The composition does not comprise ozonized honey or ozonated oil. The document also describes achieving sterility while retaining glucose oxidase activity by gamma irradiation, and addresses variability associated with manuka by removing or inactivating catalase and optionally substrate-conversion activity while enabling antimicrobial activity through hydrogen peroxide release.
Claims Coverage
The partial content provides one independent claim directed to a sterile storage-stable composition for generating antimicrobial activity for treating microbial infections. The core inventive features include an added enzyme and honey substrate combination, the honey lacking catalase and glucose oxidase activities, substantially no hydrogen peroxide with controlled free water or water activity, and exclusion of ozonized honey or ozonated oil.
Sterile storage-stable hydrogen-peroxide generating composition using enzyme plus honey substrate
A sterile storage-stable composition for generating antimicrobial activity for treating microbial infections comprising an enzyme able to convert a substrate to release hydrogen peroxide, and a honey that lacks catalase activity and lacks glucose oxidase activity and includes a substrate for the enzyme, wherein the enzyme is additional to any enzyme activity able to convert the substrate to release hydrogen peroxide that may be present in the honey.
Controlled free water or defined water activity to prevent premature hydrogen peroxide
The composition does not include sufficient free water to allow the enzyme to convert the substrate or has a water activity of 0.47 to 0.7, and comprises substantially no hydrogen peroxide.
Exclusion of ozonized honey or ozonated oil
The composition does not comprise ozonized honey or ozonated oil.
The inventive concept is a sterile storage-stable antimicrobial composition that uses an added enzyme to convert a substrate provided by honey lacking catalase and glucose oxidase activities, while maintaining substantially no hydrogen peroxide through controlled free water or water activity, and excluding ozonized honey or ozonated oil.
Stated Advantages
Reduces premature hydrogen peroxide generation during storage by limiting sufficient free water or by using a specified water activity range.
Provides reduced variability versus manuka variability by removing or inactivating catalase activity and optionally substrate-conversion activity.
Retains glucose oxidase activity after sterilization by gamma irradiation.
Enables antimicrobial potency tailoring.
Provides antimicrobial and antiviral activity outcomes as described in the document.
Provides wound-related benefits including debridement, deodorising, anti-inflammatory effects, tissue growth, and scarring reduction.
Documented Applications
Treating microbial infections using the sterile storage-stable composition for generating antimicrobial activity.
Chronic wound applications, including MRSA and antibiotic-resistant organisms.
PICC site infection.
Caesarean wound infection and surgical site infection.
Wound healing-related outcomes including debridement, deodorising, anti-inflammatory effects, tissue growth, and scarring reduction.
Antiviral activity including activity against Herpes Simplex Virus.
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