Continuous countercurrent spiral chromatography

Inventors

Shinkazh, Oleg • FEDORENKO, Dmitriy

Assignees

Chromatan Inc

Abstract

A system, module and method for continuous countercurrent spiral chromatography are disclosed. The module includes an input port for receiving an input solution, a first mixer for mixing the input solution with a recycled solution to produce a first mixed output, a stage I separator for concentrating the first mixed output to produce a stage I solid fraction, a second mixer for mixing the stage I solid fraction from the stage I separator and an optional buffer solution to produce a second mixed output, and a stage II separator for concentrating the second mixed output to produce a stage II solid fraction which exits the module. At least one separator is a spiral separator. The system includes a plurality of modules, and at least one of the plurality of modules includes a spiral separator. The method includes purifying an unpurified solution with the plurality of modules.

Core Innovation

The invention provides a continuous countercurrent spiral chromatography module in which an input solution is received at a first input port and mixed with a recycled solution to produce a first mixed output. The first mixed output is concentrated in a stage I separator to form a stage I solid fraction with a stage I liquid fraction exiting via a first output port. A second mixer mixes the stage I solid fraction with an optional buffer solution to produce a second mixed output.

The second mixed output is concentrated in a stage II separator to produce a stage II solid fraction that exits via a second output port, while a stage II liquid fraction exits via a third output port. A pump provides recycled solution that flows countercurrent to the input solution into the second input port. At least one of the stage I separator and the stage II separator is a spiral separator including a curvilinear channel arranged and disposed to generate at least one Dean vortex.

The spiral separator includes at least one inlet, at least one liquid fraction outlet, and at least one solid fraction outlet. In additional embodiments, a continuous countercurrent spiral chromatography system includes a binding step module for binding product from an unpurified product solution with a resin slurry, a washing step module for washing impurities from the resin slurry, and a regeneration step module for regenerating the resin slurry.

In further embodiments, a method purifies an unpurified solution using continuous countercurrent spiral chromatography with a plurality of modules that may include binding, washing, optionally elution, capturing purified product solution, regeneration of the resin slurry, and providing buffer solutions.

Claims Coverage

The partial content contains three independent claims: a continuous countercurrent spiral chromatography module, a continuous countercurrent spiral chromatography system with binding, washing, and regeneration, and a method for purifying an unpurified solution using continuous countercurrent spiral chromatography. Each independent claim centers on the same two-stage continuous module architecture with countercurrent recycling and spiral separation that generates Dean vortices, with system and method claims extending the architecture to process modules and purification steps.

Across the independent claims, the core claim coverage is for a continuous countercurrent spiral chromatography architecture using first and second mixers, stage I and stage II separators producing solid and liquid fractions, and a recycled solution that flows countercurrent to the input. At least one separator is a spiral separator with a curvilinear channel generating Dean vortices and providing liquid and solid outlets. The system and method independent claims further incorporate binding, washing, regeneration, and optionally elution and capture using resin slurry and buffers with the same continuous countercurrent spiral module.

Stated Advantages

Documented Applications

No documented applications found