Methods for generating animals with desirable traits
Inventors
Kasinathan, Poothappillai K. • Wei, Hong • Allan, Mark F. • Faber, David C.
Assignees
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Publication Number
US-10927411-B2
Publication Date
2021-02-23
Expiration Date
Abstract
Genetic tests, such as whole genome analysis (WGA), have been employed to identify genetically superior embryos. The disclosed methods extend in vitro culture time of embryos while awaiting results of genetic tests being performed on a portion of the same embryos. The disclosed methods also help expand the number of cells in each embryo before implantation in the recipient.
Core Innovation
The invention relates to producing a non-human mammalian progeny having a genetically desirable trait by culturing a non-human mammalian embryo in vitro to establish a candidate cell line comprising from about 300 to about 800 cells. The cultured cells are divided into two or more aliquots, and a first aliquot is subjected to a genetic analysis to test for the presence of one or more genes and/or one or more polymorphisms associated with the genetically desirable trait.
When the presence in the first aliquot identifies the candidate cell line as having the genetically desirable trait, the method proceeds to progeny production using cells from a second aliquot. If the first aliquot does not comprise the genetically desirable trait, the method repeats culturing and genetic testing with a subsequent non-human mammalian embryo until a candidate cell line having the genetically desirable trait is identified.
For bovine implementations, the method further specifies culturing a bovine embryo in vitro for 5 to 9 days, transferring the cultured embryo into a recipient female, and collecting the post-transfer embryo. Cells from the post-transfer embryo are then cultured in vitro to establish a candidate cell line, divided into aliquots, and a first aliquot is genetically analyzed for one or more genes and/or one or more polymorphisms associated with the genetically desirable trait.
Claims Coverage
The document includes two independent claims: clm-00001 and clm-00011. Across these, the inventive approach is organized around selection of candidate cell lines via genetic analysis of a first aliquot, while producing progeny from a second aliquot of the identified candidate cell line.
In vitro embryo culture to establish candidate cell line
Culturing a non-human mammalian embryo in vitro to establish a candidate cell line comprising from about 300 to about 800 cells.
Dividing candidate cell line into aliquots
Dividing the cells of the candidate cell line into two or more aliquots.
Genetic analysis of a first aliquot for genes and/or polymorphisms
Subjecting a first aliquot from the two or more aliquots to a genetic analysis to test for the presence of one or more genes and/or one or more polymorphisms associated with the genetically desirable trait, wherein the presence in the first aliquot identifies the candidate cell line as having the genetically desirable trait.
Iterative selection until candidate cell line identified
If the first aliquot does not comprise the genetically desirable trait, repeating steps (a)-(c) with a subsequent non-human mammalian embryo until the candidate cell line having the genetically desirable trait is identified.
Producing progeny using a second aliquot from the identified candidate cell line
Producing, using cells from a second aliquot from the two or more aliquots from the candidate cell line identified as having the genetically desirable trait, the non-human mammalian progeny having the genetically desirable trait.
Bovine embryo culture, recipient transfer, post-transfer collection, and candidate cell line establishment
Culturing a bovine embryo in vitro for 5 to 9 days; following the culturing of the embryo, transferring the cultured embryo into a recipient female; collecting the post-transfer embryo from the recipient female; culturing the cells from the post-transfer embryo in vitro to establish a candidate cell line.
Aliquot-based genetic analysis and repeated bovine selection
Subjecting a first aliquot from the two or more aliquots to a genetic analysis to test for the presence of one or more genes and/or one or more polymorphisms associated with the genetically desirable trait, wherein the presence in the first aliquot identifies the candidate cell line as having the genetically desirable trait; if the first aliquot does not comprise the genetically desirable trait, repeating steps (a)-(f) with a subsequent bovine embryo until the candidate cell line having the genetically desirable trait is identified.
Producing bovine progeny using a second aliquot from the identified candidate cell line
Producing, using cells from a second aliquot from the two or more aliquots from the candidate cell line identified as having the genetically desirable trait, the bovine progeny having the genetically desirable trait.
Across clm-00001 and clm-00011, the claims cover an iterative workflow in which a candidate cell line derived from in vitro embryo culture is split into aliquots, one aliquot is genetically analyzed for genes and/or polymorphisms associated with a genetically desirable trait to identify the candidate cell line, and progeny are produced from a second aliquot of the identified candidate cell line.
Stated Advantages
Documented Applications
No documented applications found
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