Deposition-conversion method for tunable calcium phosphate coatings on substrates and apparatus prepared thereof
Inventors
Harding, Jacqueline L. • Krebs, Melissa D.
Assignees
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Publication Number
US-10926000-B2
Publication Date
2021-02-23
Expiration Date
Abstract
The present invention relates to a method for in situ biomimetic mineralization of polymeric hydrogels, where the incorporated CaP phase can be selectively tuned in chemical composition and morphology to mimic bone and dental mineral. The present invention also relates to a method to coat a substrate with apatite material, the resulting product and the use of the product.
Core Innovation
A method of depositing calcium phosphate on a substrate is provided by providing a substrate comprising one of a chitosan, a cellulose, an alginate, a collagen, or combinations thereof, and preparing a coated substrate. The method includes immersing the substrate in a calcium ion solution, immersing the substrate in water, immersing the substrate into a phosphate ion solution, and alternating the immersion into the calcium ion solution and the phosphate ion solution to prepare the coated substrate.
The coated substrate is then tuned to a calcium phosphate by hydrolyzing the coated substrate in a fluid under predetermined physiological conditions to form the calcium phosphate. The calcium phosphate phase and its formation are linked to the hydrolysis step under predetermined physiological conditions, so that biomimetic mineralization is obtained by converting the coated calcium phosphate precursor into calcium phosphate.
The method further enables tuning of the calcium phosphate that is formed, including the apatite phase composition and morphology. Selection of phosphate sources, hydrolysis buffers/media, and ionic or organic additives is used to produce different particle morphologies and Ca/P ratios, including bladed, spherical, petal-like, and plate-like morphologies and calcium phosphate compositions with different Ca/P values.
Claims Coverage
The document contains one independent claim (clm-00001). It is supported by dependent claims that add hydrolysis physiological conditions, phosphate-source selections, tuning ranges, and morphology tuning.
Calcium phosphate deposition by alternating calcium and phosphate immersion and physiological hydrolysis
Providing a substrate comprising one of a chitosan, a cellulose, an alginate, a collagen, or combinations thereof; immersing the substrate in a calcium ion solution; immersing the substrate in water; immersing the substrate into a phosphate ion solution; alternating the immersion of the substrate into the calcium ion solution and the phosphate ion solution to prepare a coated substrate; and tuning the coated substrate to a calcium phosphate by hydrolyzing the coated substrate in a fluid under predetermined physiological conditions to form the calcium phosphate.
Overall claim coverage is centered on alternating immersion in calcium ion solution and phosphate ion solution to prepare a coated substrate, followed by hydrolyzing the coated substrate under predetermined physiological conditions to form and tune calcium phosphate.
Stated Advantages
Tuning of the calcium phosphate formed by controlling the hydrolyzing step under predetermined physiological conditions.
Tuning of calcium phosphate composition and morphology, including different particle morphologies and Ca/P ratios.
Documented Applications
Dental applications.
Bone/orthopedic uses and implant coatings.
Regenerative or biomimetic biodegradable grafts.
Anti-biofouling.
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