Process for functionalizing a biocompatible polymeric bead, the functionalized beads, and the beads produced thereby
Inventors
Golobish, Thomas • Guliashvili, Tamaz • Capponi, Vincent
Assignees
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Publication Number
US-10889665-B2
Publication Date
2021-01-12
Expiration Date
Abstract
The invention involves functionalizing polymeric beads, such as DVB beads, to add an epoxide or aldehyde group. The resulting beads are useful in various applications.
Core Innovation
The invention describes porous divinylbenzene or styrene-divinylbenzene beads with surface epoxide groups formed through in situ generation of peracetic acid from acetic acid and hydrogen peroxide. A biological molecule can attach to the epoxide groups, and the epoxide groups can also be converted to aldehyde groups. The claimed beads have pores with an average diameter below 200 Angstroms.
The supplied partial content does not include the background section, so the problem addressed there is not explicitly described in patent. The independent claim covers a process for making divinylbenzene polymeric beads with surface epoxide groups and an attached biological molecule. The partial content also describes steam stripping to reduce residual reagents and an assay involving lactate dehydrogenase attached to epoxy-functionalized beads.
Claims Coverage
The claims coverage includes one inventive feature covering a process for producing porous divinylbenzene beads with surface epoxide groups and an attached biological molecule.
Epoxide-functionalized beads with an attached biological molecule
The claim covers porous polymeric beads comprising monomeric divinylbenzene and pores with an average diameter below 200 Angstroms. Acetic acid and hydrogen peroxide form surface epoxide groups, to which a biological molecule attaches. Dependent claims further describe conversion of epoxide groups to aldehyde groups, a bead diameter range of 50–150 μm, and steam stripping to remove unreacted divinylbenzene.
The claim centers on porous divinylbenzene beads bearing surface epoxide groups and an attached biological molecule, with further elements detailing aldehyde conversion, specified bead diameter ranges, and steam stripping.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Attaching biological molecules, including lactate dehydrogenase, to epoxy-functionalized porous beads, and performing an assay comparing the activity of lactate dehydrogenase-conjugated beads with unconjugated beads.
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