Closed fluid receiving and sampling container
Inventors
Assignees
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Publication Number
US-10843186-B1
Publication Date
2020-11-24
Expiration Date
Abstract
A closed fluid receiving and sampling container that enables transfer of valuable reaction liquid to a receptacle without risking loss of sterility. The sampling container has a dip tube subassembly with a shorter inlet tube bent towards the wall of the receptacle to prevent or reduce foaming, and a longer outlet tube used to drain the waste liquid once the magnetic beads are trapped by the magnet. The dip tube subassembly is injection molded in one piece and provides a sealed lid also with a vent tube therethrough to enable filling and draining the receptacle without removing the lid, thus keeping the process aseptic. The sampling container is especially useful in the context of magnetic bead separation processes.
Core Innovation
The invention relates to a closed chemical or biological processing assembly for receiving and sampling liquid. The assembly includes a receptacle having a closed lower end and an open top end, and a lid subassembly configured to seal over the open top end of the receptacle. The lid subassembly includes an inlet tube extending downward from the lid in communication with an inlet connector projecting upward from the lid, and an outlet tube extending downward from the lid in communication with an outlet connector projecting upward from the lid, with the outlet tube extending farther downward than the inlet tube and a vent connector projecting upward from the lid and having an opening to a lower side of the lid.
The closed system further includes a cap ring configured to be secured around the open top end of the receptacle to retain the lid over the open top end. A reactor contains a liquid reaction medium with a reactor outlet and a reactor outlet tube attached to the inlet connector of the subassembly. The assembly provides a system for receiving and sampling liquid within a closed framework while enabling transfer of liquid reaction medium through reactor connections.
A stand having a plurality of stations is adapted for holding the receptacle, and the stand has magnets or is magnetized on an inner vertical wall at each of the stations. The receptacle contains magnetically-attractive beads configured to attract a desirable component of the liquid reaction medium, such that the desirable component may be isolated on the beads and held against an inner wall of the receptacle adjacent the inner vertical wall of a respective station. The assembly further includes closed-system sampling and flow-path switching structures, including a three-way stopcock attached to the outlet connector, a return tube connected to a junction of the three-way stopcock and to a return inlet to the reactor, and a sampling tube connected to a junction of the three-way stopcock.
Claims Coverage
Two independent claims are present. They cover a closed system for receiving and sampling liquid with a sealed lid subassembly including inlet and outlet tubes extending into the receptacle, reactor connections, and closed flow-path switching; one independent claim further specifies a magnetized multi-station stand for magnetic-bead isolation.
Sealed receptacle with inlet, outlet, and vent connectors
A lid subassembly configured to seal over the open top end of the receptacle, including an inlet tube extending downward in communication with an inlet connector projecting upward, an outlet tube extending downward in communication with an outlet connector projecting upward, and a vent connector projecting upward from the lid and having an opening to a lower side of the lid; the outlet tube extends farther downward than the inlet tube.
Cap ring for retaining the lid
A cap ring configured to be secured around the open top end of the receptacle to retain the lid over the open top end.
Reactor connection to inlet connector of the closed system
A reactor containing a liquid reaction medium with a reactor outlet and a reactor outlet tube attached to the inlet connector of the subassembly.
Magnetized multi-station stand for bead isolation
A stand having a plurality of stations adapted for holding the receptacle, the stand having magnets or being magnetized on an inner vertical wall at each station; the receptacle contains magnetically-attractive beads configured to attract a desirable component of the liquid reaction medium, such that the desirable component may be isolated on the beads and held against an inner wall of the receptacle adjacent the inner vertical wall of a respective station.
Three-way stopcock with return and sampling tubes
A three-way stopcock attached to the outlet connector of the closed system, a return tube connected to a junction of the three-way stopcock and to a return inlet to the reactor, and a sampling tube connected to a junction of the three-way stopcock.
The inventive features are a sealed lid subassembly with inlet, outlet, and vent connectors, retention of the lid by a cap ring, reactor connection to the receptacle, closed-system routing with a three-way stopcock and return and sampling tubes, and a magnetized multi-station stand for holding magnetically-attractive beads to isolate a desirable component.
Stated Advantages
Enables isolation of a desirable component of the liquid reaction medium on magnetically-attractive beads held against an inner wall at a respective station.
Documented Applications
Chemical or biological processing using a closed system for receiving and sampling a liquid reaction medium with reactor connections and sampling/diverter routing via a three-way stopcock.
Magnetic bead separation in a receptacle held in a multi-station magnetic stand, where a desirable component is isolated on beads and held against an inner wall adjacent the magnetized station.
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