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Publication Number
US-10793920-B2
Publication Date
2020-10-06
Expiration Date
Abstract
The invention relates to a method of identifying a specific yeast species in patient tissue or body fluid. The method comprises the steps of extracting and recovering DNA of the yeast species from the patient tissue or body fluid, amplifying the DNA, hybridizing a probe to the DNA to specifically identify the yeast species, and specifically identifying the yeast species. The invention also relates to a method of identifying a yeast mycotoxin in patient tissue or body fluid. The method comprises the steps of extracting and recovering the yeast mycotoxin from the patient tissue or body fluid, contacting the yeast mycotoxin with an antibody directed against the yeast mycotoxin, and identifying the yeast myocotoxin. Both of these methods can be used to determine if a patient is at risk for or has developed a disease state related to a yeast infection, and to develop an effective treatment regimen for the patient.
Core Innovation
The invention provides a method of identifying a specific yeast species in patient tissue or body fluid by extracting and recovering DNA of the yeast species from the patient tissue or body fluid, amplifying the DNA, and hybridizing a probe to the DNA to specifically identify the yeast species. The probe sequence is selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 10, a full complement of the sequence of SEQ ID NO: 4, and a full complement of the sequence of SEQ ID NO: 10. The method specifically identifies the yeast species as Candida glabrata or Candida tropicalis.
The invention further grounds the identification by using a specific DNA target for the amplification. In particular implementations, the method amplifies internal transcribed spacer regions of nuclear ribosomal DNA, and an amplification step is performed using primers that hybridize to the DNA. Additional claim refinements specify combinations of probe and primer sequence identifiers, including a probe with specific sequence identifiers together with forward and reverse primers using specified sequence identifiers.
The invention also describes extending the species identification scope to other yeast species. Dependent claim refinements identify other yeast species selected from Candida albicans and Candida kruseii. The overall concept is therefore species-specific identification of yeast from patient tissue or body fluid using DNA extraction, amplification, and probe-based hybridization using sequences defined by SEQ ID NO selections and their full complements.
Claims Coverage
The document includes one independent claim. The independent claim covers a probe-sequence-selected DNA hybridization approach for identifying Candida glabrata or Candida tropicalis in patient tissue or body fluid using extracted yeast DNA and amplification.
Species-specific yeast identification from patient tissue or body fluid using extracted and recovered yeast DNA
A method including extracting and recovering DNA of the yeast species from the patient tissue or body fluid, amplifying the DNA, and specifically identifying the yeast species.
Probe sequence selected from defined SEQ ID NO or full complements for species discrimination
Hybridizing a probe to the amplified DNA to specifically identify the yeast species, wherein the probe sequence is selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 10, a full complement of SEQ ID NO: 4, and a full complement of SEQ ID NO: 10.
Specific identification limited to Candida glabrata or Candida tropicalis
Specifically identifying the yeast species, wherein the yeast species is Candida glabrata or Candida tropicalis.
Overall, the claim coverage centers on extracting yeast DNA from patient tissue or body fluid, amplifying the DNA, and hybridizing a probe whose sequence is selected from defined SEQ ID NO: 4 and SEQ ID NO: 10 or their full complements to specifically identify Candida glabrata or Candida tropicalis, with dependent claims narrowing amplification targets and expanding to additional Candida species.
Stated Advantages
Documented Applications
No documented applications found
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