Sorbent material for separating bio-macromolecules
Inventors
O'Halloran, Jonathan James • Hedley, Joseph H. • Whitehouse, Sam • FLANAGAN, Marc • Tyson, John • DIXON, Rachael • BHATT, Sam • CROZIER, Emily • Howell, Chris
Assignees
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Publication Number
US-10792640-B2
Publication Date
2020-10-06
Expiration Date
Abstract
A sorbent material is disclosed for the one-step separation of bio-macromolecules in a single pass extraction of DNA from complex mixtures of molecules and chemicals. In one embodiment, the sorbent material comprises a silanized material at least partially coated or formed with a polymer selected from the group consisting of a poly(aryl methacrylate), a poly(aryl acrylate), a poly(heteroaryl methacrylate, a poly(heteroaryl acrylate) and a copolymer thereof.
Core Innovation
The invention relates to a sorbent material for separating bio-macromolecules by retaining proteins and other non-nucleic acids but not nucleic acids. The sorbent material comprises a silanized material that is at least partially coated or formed with a polymer with a single repeating unit. The polymer is selected from poly(aryl methacrylate), poly(aryl acrylate), poly(heteroaryl methacrylate), and poly(heteroaryl acrylate).
The described sorbent discriminates between proteins and nucleic acids using the combination of a silanized inorganic substrate and a single repeating-unit polymer chemistry. The partial content further ties discrimination to polymer selection, surface wettability, and microstructural parameters of the silanized inorganic material.
In addition to the polymer selection, the partial content describes performance relationships linked to surface wettability and polymer aromatic content promoting protein adsorption while repelling DNA. It further states that pore size and active surface area, together with average interstitial spacing and particle-size uniformity, affect DNA/protein discrimination. The polymer-coated silanized inorganic material is described in multiple forms including silanized silica particles, fibers, porous silicon, and silanized silica membrane or monolith structures.
Claims Coverage
The independent claim provides the core separation function and the key structural limitation of a silanized material coated or formed with a polymer having a single repeating unit; it identifies multiple inventive features. Dependent refinements further specify the silanized substrate form, quantitative surface/microstructure parameters, and named polymer recurring units.
Protein-retaining, nucleic-acid-repelling sorbent for biomacromolecule separation
A sorbent material that will retain proteins and other non-nucleic acids but not nucleic acids, for separating bio-macromolecules.
Silanized material at least partially coated or formed with a single repeating-unit polymer
The sorbent material comprising a silanized material at least partially coated or formed with a polymer with a single repeating unit.
Polymer selected from aryl and heteroaryl methacrylates/acrylates
The polymer is selected from the group consisting of a poly(aryl methacrylate), a poly(aryl acrylate), a poly(heteroaryl methacrylate), and a poly(heteroaryl acrylate).
Silanized silica forms (particles, fibers, monolithic membrane/structures)
The silanized material is a silanized inorganic material selected from a silanized silica particle, a silanized silica fiber, or a silanized silica monolithic membrane/structure.
Silanized silica particle pore size range
Silanized silica particles with an average pore size between 1 nm and 100 nm.
Sorbent surface area range
A surface area between 0.1 m2 and 130 m2.
Particulate article with average interstitial distance threshold
A particulate article that includes the sorbent material and has an average interstitial distance greater than about 10 nm.
Named single repeating-unit polymer recurring units
The polymer includes a recurring unit chosen from benzyl methacrylate, anisolemethyl methacrylate, phenylethanol methacrylate, pyridinemethyl methacrylate, or naphthalenemethyl methacrylate.
Overall, the claim set centers on a silanized inorganic sorbent coated or formed with a single repeating-unit polymer selected from aryl/heteroaryl methacrylates and acrylates, configured to retain proteins and other non-nucleic acids while not retaining nucleic acids, with dependent features constraining silica form, pore size, surface area, particulate interstitial distance, and named polymer recurring units.
Stated Advantages
Retains proteins and other non-nucleic acids but not nucleic acids for separating bio-macromolecules.
Documented Applications
Separating bio-macromolecules using a single-pass extraction/purification of DNA from complex mixtures such as blood lysate, with reported DNA/protein fraction comparisons.
Integration into a dual-processing device that lyses a sample and passes lysate through the sorbent filter for rapid DNA extraction/purification, with comparative extraction data versus alternative devices.
Using the sorbent in DNA extraction cassettes and spin columns, with comparative DNA/protein fraction reporting.
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