Closed disposable multiple sterile blood bag system for fractionating blood with the corresponding method

Inventors

Vincent, Emmanuel • BOHEC, Pierre • GACHELIN, Jeremie

Assignees

Aenitis Technologies SAS

Abstract

Disclosed is a multiple bag system for fractionating blood, the system including a fluid collecting bag including at least one outlet port; at least first and second sampling bags, each including at least one inlet port and at least one outlet port; and a fluid transfer unit to transfer fluid from the collecting bag to the sampling bags. The fluid transfer unit includes an acoustic sorter. Also disclosed is a method for fractionating blood into blood products.

Core Innovation

The invention relates to a closed disposable multiple sterile blood bag system for fractionating blood. The system includes a fluid collecting bag with at least one outlet port, and first and second sampling bags, each with at least one inlet port and at least one outlet port. First means transfer fluid from the fluid collecting bag to the sampling bags through a fluid-transfer channel with an acoustic wave generator arrangement.

The fluid-transfer channel extends along a longitudinal axis and has a cross section with a width measured along a first transverse axis and a thickness measured along a second transverse axis perpendicular to the first transverse axis. At least one wall is arranged for receiving an acoustic wave generator, and the thickness of the channel and the acoustic wave generator cooperate to generate a volumetric standing wave over the thickness of the channel for sorting and fractionating a fluid. At least one inlet and first and second outlets are provided in fluid communication with the channel, with the first outlet separated from the second outlet on the second transverse axis.

The outlet port of the fluid collecting bag is fluidly and sterilely connected to the inlet of the transfer means, and the first and second outlets are fluidly and sterilely connected to the inlet ports of the respective sampling bags. A method for high throughput preparation of blood products is also provided, applying an acoustic field inside the channel using an acoustic wave generator configured to generate an acoustic force field over the thickness of the channel for sorting and fractionating a fluid. The acoustic field contains volumetric acoustic standing waves and not surface acoustic waves, and the method collects blood cells in one sampling bag and blood plasma in the other sampling bag.

In additional embodiments, buffer bags containing a buffer medium are included and connected to provide a buffer to the fluid-transfer inlet, and further sampling and transfer stages are used. The system extends to collecting additional blood product fractions, including red blood cell concentrate and platelet concentrate, and also white blood cell concentrate. The channel thickness and the acoustic wave generator are constrained by wavelength-based relationships, and a reflector wall is characterized by an acoustic reflection coefficient range.

Claims Coverage

The document provides two independent claims: one directed to a closed disposable multiple sterile blood bag system for fractionating blood, and one directed to a method for high throughput preparation of blood products. Each independent claim includes a core set of inventive features centered on sterile closed bag connections and acoustic-field-driven sorting/fractionation using volumetric standing waves across a fluid-transfer channel thickness, with further optional refinements in dependent claims.

Across both independent claims, the inventive concept is the same: a closed disposable sterile multiple blood bag setup with sterile fluid connections combined with an acoustic-wave-generator/channel arrangement that generates volumetric acoustic standing waves (not surface acoustic waves) over the channel thickness to sort and fractionate whole blood into different sampling bags.

Stated Advantages

Documented Applications