Highly active silica magnetic nanoparticles for purifying biomaterial and preparation method thereof
Inventors
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Assignees
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BioneerBioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Bioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Publication Number
US-10724031-B2
Publication Date
2020-07-28
Expiration Date
Abstract
The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
Core Innovation
The invention relates to silica-coated magnetic nanoparticles consisting of magnetic nanoparticles completely coated with silica. The nanoparticles have a silica content of 0.5-2.5 wt %, a particle size of 420-450 nm, a coating thickness of 2-13 nm, and a hydroxyl group on the silica surface.
A divisional invention is described for making highly active silica-coated magnetic nanoparticles by ultrasonically dispersing iron oxide magnetic nanoparticles in a distilled water/alcohol solvent using an acid-base catalyst and a water-soluble hydrophobic solvent. Silane is added to form the silica coating, with the coating thickness controlled to fall within the stated range.
The resulting silica-coated magnetic nanoparticles are used as nucleic-acid-binding carriers in chaotropic-reagent binding/elution workflows. The disclosure further describes compositions and kits for nucleic acid purification/diagnosis, including binding, washing, and elution solutions, and the use of a magnet to perform binding and separation.
Claims Coverage
The document contains one independent claim directed to the physical nanoparticle structure, with multiple dependent claims that narrow preparation and provide compositions and kits for nucleic-acid purification or detection. The independent claim centers on specified silica content, particle size, coating thickness, complete silica coating, and a hydroxyl-group-bearing silica surface.
Silica-coated magnetic nanoparticles with hydroxylated silica surface and specified silica/size/thickness
Silica-coated magnetic nanoparticles consisting of magnetic nanoparticles completely coated with silica, wherein the silica content is 0.5-2.5 wt %, a particle size is 420-450 nm, a coating thickness is 2-13 nm, and the surface of the silica has a hydroxyl group thereon.
Preparation by ultrasonically dispersing with acid-base catalyst, hydrophobic solvent, and silane addition
Silica magnetic nanoparticles prepared by ultrasonically dispersing magnetic nanoparticles with an acid-base catalyst and a hydrophobic organic solvent in a solvent mixture of distilled water and an alcohol, and then adding a silane.
Nucleic-acid purification or detection composition containing the nanoparticles
A composition for purifying or detecting nucleic acid that comprises the silica-coated magnetic nanoparticles.
Nucleic-acid purification or detection kit containing the nanoparticles
A kit for purifying or detecting nucleic acid that comprises the silica-coated magnetic nanoparticles.
Across the claim set, the core inventive concept is the specified structure of silica-coated magnetic nanoparticles, with dependent coverage extending to an ultrasonically dispersed, acid-base-catalyzed silane-coating preparation approach and to compositions and kits for nucleic-acid purification or detection.
Stated Advantages
Higher binding capacity for DNA compared with conventional micro silica magnetic particles (about 2.7× reported).
Documented Applications
Nucleic-acid purification and diagnosis using binding, washing, and elution buffers with chaotropic reagents and magnetic separation.
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