Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof
Inventors
Siciliano, Nicholas A. • Bouliane, Martin Joseph • Leong, Louis
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-10705084-B2
Publication Date
2020-07-07
Expiration Date
Abstract
Devices and methods for the detection of analytes are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.
Core Innovation
The invention provides an analyte detection device comprising a housing with an inlet and a channel system that transports fluid from the inlet to first and second analyte detection membrane systems. Each analyte detection membrane system includes a conjugate pad, an optional permeable membrane, a test membrane, and an absorbent member arranged in an order, with substantially portions of the conjugate pad, permeable membrane, test membrane, and absorbent member arranged substantially parallel to each other.
The device includes first and second analyte detection membrane systems that are substantially parallel and are capable of being compressed. A first force member is in contact with a first force actuator outlet and contacts the absorbent member of the first analyte detection membrane system, where engagement applies pressure substantially perpendicular to the first analyte detection membrane system. A second force member is in contact with a second force actuator outlet and contacts the absorbent member of the second analyte detection membrane system, where engagement applies pressure substantially perpendicular to the second analyte detection membrane system.
A movable locking member contacts the first force member and the second force member, and flexible or fixed attachment members attached to the movable locking member connect to the conjugate pads of the first and second analyte detection membrane systems. The described arrangement supports parallel analyte detection from a single inlet and mechanical compression of each membrane stack via separate force members, while fluid transport is directed by the channel system to the first and second analyte detection membrane systems.
Claims Coverage
The document includes a primary independent claim that sets out the overall device architecture, including parallel analyte detection membrane systems and independently engageable, substantially perpendicular pressure application via two force members and actuator outlets. Dependent claims refine mechanical actuation details, channel architecture, and analyte/capture reagent specificity, including food-borne pathogen analyte embodiments and labeled capture reagent conjugates.
Parallel dual analyte detection membrane systems within a housing
A housing with an inlet, first and second analyte detection membrane systems in an order of conjugate pad, optional permeable membrane, test membrane, and absorbent member, where at least a portion of each of the conjugate pad, permeable membrane, test membrane, and absorbent member are substantially parallel to each other.
Channel system transporting fluid to parallel membrane systems
A channel system or membrane transports fluid from the inlet to the first and second analyte detection membrane systems.
Movable locking member with dual force members for perpendicular compression
A movable locking member contacting the first force member and the second force member, where the first force member contacts the absorbent member of the first analyte detection membrane system and when engaged applies pressure substantially perpendicular to the first analyte detection membrane system, and where the second force member contacts the absorbent member of the second analyte detection membrane system and when engaged applies pressure substantially perpendicular to the second analyte detection membrane system.
Attachment members coupling movable locking member to conjugate pads
A first flexible or fixed attachment member attached to the movable locking member and the conjugate pad of the first analyte detection membrane system, and a second flexible or fixed attachment member attached to the movable locking member and the conjugate pad of the second analyte detection membrane system.
Overall claim coverage emphasizes a device architecture combining substantially parallel, compressible first and second analyte detection membrane systems with a channel system that feeds both systems from a single inlet, and a dual-force, movable-locking actuation arrangement that applies pressure substantially perpendicular to each membrane stack.
Stated Advantages
Fast detection is demonstrated with detection times stated as approximately 30–60 seconds to approximately 4 minutes depending on target.
Species specificity is demonstrated, with stated no cross reactivity for E. coli O157:H7, Salmonella enterica, and Campylobacter jejuni.
Multiplex detection from a single sample is demonstrated.
Robustness against contaminants and non-pathogenic strains is stated.
Limits of detection are stated in a range of approximately 10^3 to 10^4 CFU/mL.
Documented Applications
Food-borne pathogen detection using the described analyte detection devices and assays, with examples including E. coli O157:H7, Salmonella enterica, and Campylobacter jejuni.
Multiplex detection from a single sample using multiple analyte detection membrane systems.
Interested in licensing this patent?