Rapid screening assay for qualitative detection of multiple febrile illnesses
Inventors
Esfandiari, Javanbakhsh • Gunasekera, Angelo H.
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-10690667-B2
Publication Date
2020-06-23
Expiration Date
Abstract
A single-use multiplex, or assay, screening test for the detection of one or more of a plurality of unrelated febrile illnesses is provided. The febrile illnesses for which the test is designed are unrelated, in that the illnesses may be caused, by way of example, by infection from viruses, bacterium and/or parasites; by infection from viruses, bacterium, parasites or other contagions that are animal borne; by infection from viruses, bacterium, parasites, or other contagions that can be aerosolized for transmission; by infection from viruses, bacterium, parasites or other contagions that are transmitted from direct contact; by infection from viruses, bacterium, parasites or other contagions that are generally transmitted in the tropics and/or subtropics; and/or by infection from a virus, bacteria, parasite sharing one or more related feature and which causes a febrile illness. The assay test provides rapid results to a point of care center or other facility requiring such results to facilitate treatment and or containment of the illnesses.
Core Innovation
The invention provides a test device for the qualitative detection from a liquid sample of at least one febrile illness from a plurality of pathogenically unrelated febrile illnesses. The device includes sorbent materials arranged to define migration paths for the solution and for conjugates, with a first sorbent material receiving the solution and having a plurality of conjugates having antigens or antibodies with attached visible color markers deposited thereon.
A second sorbent material is separate from the first sorbent material and defines a separate migration path for the liquid sample. A test site includes a plurality of separately located immobilized ligand-binding mechanisms for receiving the liquid sample, and the plurality includes a first mechanism and a second mechanism.
The first mechanism binds to a viral infection antigen or antibody, and the second mechanism binds to a bacterial infection antigen or antibody or to a parasitic infection antigen or antibody. The liquid sample laterally flows from the second location to the test site without immediately wetting the test site upon application, and a single control line spaced from the test site has anti-conjugate antibodies so that the plurality of conjugates bind to the anti-conjugate antibodies at the single control line.
Claims Coverage
The partial content identifies one independent claim. The inventive features center on a qualitative multiplex test device with two separate sorbent migration paths, multiple immobilized ligand-binding mechanisms for pathogenically unrelated febrile illnesses, a single spaced control line with anti-conjugate antibodies, and a timing constraint so the test site is not immediately wetted upon application.
Qualitative multiplex febrile illness detection with pathogenically unrelated ligand targets
A test device for the qualitative detection from a liquid sample of at least one febrile illness from a plurality of pathogenically unrelated febrile illnesses, where immobilized ligand-binding mechanisms bind corresponding ligands for the pathogenically unrelated febrile illnesses.
Dual separate sorbent materials defining separate migration paths
A first sorbent material having a first location for receiving the solution and defining a first migration path for the solution and conjugates, and a second sorbent material separate from the first sorbent material having a second location for receiving the liquid sample and defining a second migration path separate from the first migration path.
Separate conjugate and immobilized binding site architecture with viral and bacterial/parasitic mechanisms
Conjugates having antigens or antibodies with attached visible color markers deposited on the first sorbent material, and a test site with a plurality of separately located immobilized ligand-binding mechanisms including a first mechanism binding viral infection antigen or antibody and a second mechanism binding bacterial infection antigen or antibody or parasitic infection antigen or antibody.
Time-controlled lateral flow so test site is not immediately wetted
Time is required for the liquid sample to laterally flow from the second location to the test site such that the liquid sample does not immediately wet the test site upon application.
Single control line with anti-conjugate antibodies
A single control line spaced from the test site and having anti-conjugate antibodies such that the plurality of conjugates will bind to the anti-conjugate antibodies at the single control line.
The main inventive structure is a qualitative multiplex febrile-illness test device using two separate sorbent materials and separate migration paths, multiple separately located immobilized ligand-binding mechanisms that include viral and bacterial/parasitic binding categories, a timing constraint so the test site is not immediately wetted, and a single spaced control line with anti-conjugate antibodies.
Stated Advantages
Documented Applications
No documented applications found
Interested in licensing this patent?