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Publication Number
US-10626432-B2
Publication Date
2020-04-21
Expiration Date
Abstract
A composition is provided. The composition comprises an enzyme and a ruthenium- or osmium-based electron transfer agent, wherein the ruthenium- or osmium-based electron transfer agent is a complex [M(A)x(B)y](X)n wherein M is ruthenium or osmium; A is an amine ligand; each B is a ligand different to A; x is an integer selected from 1 to 5; y is an integer selected from 1 to 5; x+y is 6 or 8; n is an integer selected from 1 to 6; and X is any suitable counterion.
Core Innovation
The invention concerns an electrochemical test device for determining a concentration of an analyte in a fluid sample. The device includes a set of electrodes including a working electrode and sensing chemistry for the working electrode.
The sensing chemistry comprises a diaphorase and a ruthenium- or osmium-based electron transfer agent. The electron transfer agent is provided as a complex of formula I: (M(A)x(B)y)(X)n, where M is ruthenium or osmium, A is an amine ligand, each B is a ligand different to A, x and y are integers such that x+y is 6 or 8, n is an integer from 1 to 6, and X is any suitable counterion.
The sensing chemistry is disposed on the working electrode in two layers. A layer adjacent the working electrode comprises the diaphorase, and the multi-layer sensing chemistry concept is used together with the ruthenium- or osmium-based electron transfer agent.
Claims Coverage
The provided material contains one independent claim directed to an electrochemical test device with specified sensing chemistry, plus dependent claim refinements that narrow or extend the electron transfer agent and sensing components. The core inventive elements include the diaphorase plus Formula I ruthenium-/osmium electron transfer agent, and the two-layer placement of the sensing chemistry with the diaphorase in the layer adjacent the working electrode.
Two-layer diaphorase and Formula I electron transfer agent sensing chemistry
An electrochemical test device for determining a concentration of an analyte in a fluid sample with a working electrode and sensing chemistry, wherein the sensing chemistry comprises a diaphorase and a ruthenium- or osmium-based electron transfer agent as a complex of formula I (M(A)x(B)y)(X)n, and wherein the sensing chemistry is disposed on the working electrode in two layers, further wherein a layer adjacent the working electrode comprises the diaphorase.
Formula I ruthenium or osmium electron transfer agent with coordination constraints
The electron transfer agent in the sensing chemistry is a complex of formula I where M is ruthenium or osmium, A is an amine ligand, each B is a ligand different to A, x is an integer selected from 1 to 5, y is an integer selected from 1 to 5, x+y is 6 or 8, n is an integer selected from 1 to 6, and X is any suitable counterion.
Optional standard redox potential constraint for the electron transfer agent
The device is configured so that the ruthenium- or osmium-based electron transfer agent has a standard redox potential within -0.52 to 0.35 V.
Optional counterion selection for the electron transfer agent
The device is configured so that the counterion X is selected from F-, Cl-, Br-, I-, or PF6-.
Optional named ruthenium complexes for the electron transfer agent
The device uses a ruthenium complex selected from [RuIII(NH3)5(pyridine)]X, [RuIII(NH3)5(4-methyl pyridine)]3PF6, and [RuIII(NH3)5Cl]2Cl, where X is an unspecified counterion in the claim set and specific counterions are indicated for some examples.
Optional diaphorase selection between NADH and NADPH dehydrogenase
The device is configured so that the diaphorase is either NADH dehydrogenase or NADPH dehydrogenase.
Optional inclusion of glucose oxidase in the sensing chemistry
The sensing chemistry further includes glucose oxidase.
Overall, the claims cover an electrochemical test device where the working electrode has two-layer sensing chemistry combining a diaphorase with a ruthenium- or osmium-based electron transfer agent defined by Formula I, with refinements that optionally constrain redox potential, constrain counterions, specify example ruthenium complexes, select the diaphorase as NADH or NADPH dehydrogenase, and add glucose oxidase.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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