Compositions and methods for controlling plant-parasite nematode
Inventors
Riggs, Jennifer Lynn • Cala, Eder Leonardo Sastoque • Kloepper, Joseph W. • Lawrence, Katheryn Kay Scott • Russi, Juan David Castillo
Assignees
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Publication Number
US-10506813-B2
Publication Date
2019-12-17
Expiration Date
Abstract
An composition having anti-nematodal activity derived from Bacillus firmus bacteria selected from the group consisting of isolated biosurfactant, isolated protease enzyme, isolated amylase, isolated lipase, and isolated cellulase, is disclosed. The disclosure also provides for methods of making the same, methods of controlling plant-parasite nematode, and methods of protecting plants.
Core Innovation
The invention relates to anti-nematodal entities derived from Bacillus firmus, including an isolated biosurfactant and isolated enzymes obtained from a Bacillus firmus culture. The entities include at least one acid precipitate from the culture medium in which Bacillus firmus was grown, and the composition comprises at least one isolated biosurfactant derived from Bacillus firmus together with at least one acid precipitate obtained from the culture medium.
The document emphasizes anti-nematodal activity against plant-parasitic nematodes, including Rotylenchulus (Rotylenchulus) reniformis. It describes evaluation outcomes such as reduced egg hatch and paralysis of second-stage juveniles.
The anti-nematodal biosurfactant and/or enzymes are formulated and applied in plant-related contexts, including plants, seeds, and soil, to provide nematode control and protection. Greenhouse outcomes are reported, including effects of seed treatment, with controls including imidacloprid, and testing for systemic resistance induction.
Claims Coverage
The independent claim covers a composition with anti-nematodal activity defined by two components: at least one isolated biosurfactant derived from Bacillus firmus and at least one acid precipitate obtained from the Bacillus firmus culture medium. The independent claim is refined by dependent claims that introduce additional inventive feature constraints, including the manner of preparing the acid precipitate, concentration-related constraints for the biosurfactant, and targeting Rotylenchulus reniformis.
Anti-nematodal composition with isolated Bacillus firmus biosurfactant and acid precipitate
A composition having anti-nematodal activity comprising at least one isolated biosurfactant derived from Bacillus firmus and at least one acid precipitate from a culture medium in which the Bacillus firmus was grown.
Bacillus firmus acid precipitate generated by adding an acid
The at least one acid precipitate is generated by adding an acid to the culture medium containing Bacillus firmus to generate the precipitate, then isolating the precipitate from the culture medium.
Mineral acid precipitation with final pH 2.0
The at least one acid precipitate is generated by adding a mineral acid to the culture medium to obtain a final pH of 2.0.
Isolation of acid precipitate by centrifugation and solvent extraction
The at least one acid precipitate is isolated from the culture medium by centrifugation and extracted using dichloromethane or methanol.
Biosurfactant concentration greater than 0.5 ppm
The composition has a biosurfactant concentration greater than 0.5 ppm.
Target nematode Rotylenchulus reniformis
The plant-parasitic nematode is Rotylenchulus reniformis.
Overall, claim coverage is centered on an anti-nematodal composition defined by a combination of isolated Bacillus firmus biosurfactant and an acid precipitate from the Bacillus firmus culture medium, with refinements that specify how the acid precipitate is generated, how it is isolated, a biosurfactant concentration threshold, and targeting the plant-parasitic nematode Rotylenchulus reniformis.
Stated Advantages
Anti-nematodal activity against plant-parasitic nematodes, including reduced egg hatch and paralysis of second-stage juveniles.
Targeted nematode spectrum with emphasis on Rotylenchulus (Rotylenchulus) reniformis.
Greenhouse outcomes showing effects of seed treatment for nematode control and protection, with systemic resistance induction testing reported.
Documented Applications
Application to plants, seeds, and soil for nematode control and protection, including seed treatment contexts evaluated in greenhouse trials with imidacloprid as a control.
Testing against plant-parasitic nematodes, with emphasis on Rotylenchulus (Rotylenchulus) reniformis, including in vitro and greenhouse evidence based on egg hatch and second-stage juvenile outcomes.
Assessment for systemic resistance induction in the context of plant and seed treatment.
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