Device for detection of analytes and uses thereof
Inventors
Siciliano, Nicholas A. • Bouliane, Martin Joseph
Assignees
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Publication Number
US-10495638-B2
Publication Date
2019-12-03
Expiration Date
Abstract
Devices and methods for the detection of antigens are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.
Core Innovation
The invention relates to a device for detecting an analyte using a housed antigen detection membrane system designed for vertical flow. The device includes a conjugate pad, a test membrane, and an absorbent member arranged between first inner member and second inner member that are in contact with each other, and an inlet in fluid contact with the conjugate pad is provided by the outer member and first inner member.
At least a portion of each of the conjugate pad, test membrane, and absorbent member are substantially parallel to each other. The analyte detection membrane system comprising the test membrane and the absorbent member is compressed between the first inner member and the second inner member, while the conjugate pad is not compressed between the first and second inner members.
The disclosed configuration enables vertical flow by using a force member to compress the stacked membrane system while leaving the conjugate pad uncompressed. The device can include first and second outer members with an inlet, removable or movable layers or tabs that expose or move the conjugate pad relative to the rest of the membrane system, and integration into a system or kit that can include controls, buffer, and a sample collector.
Claims Coverage
The independent claim covers one main device concept built from inventive structural relationships between outer members, inner members, a conjugate pad, a test membrane, and an absorbent member, with a compressed vertical-flow membrane system while excluding the conjugate pad from compression. Dependent claims refine the device structure and extend coverage to optional kit components and specific material selections.
Inlet-coupled conjugate pad with compressed test-and-absorbent membrane system
A device for detecting an analyte comprising first outer member and second outer member, a conjugate pad, a first inner member and a second inner member in contact with each other, an inlet in fluid contact with the conjugate pad, and between the first and second inner members an analyte detection membrane system comprising, in order, a test membrane and an absorbent member.
Substantially parallel pad and compressed membrane system while leaving conjugate pad uncompressed
At least a portion of each of the conjugate pad, test membrane, and absorbent member are substantially parallel to each other, and the analyte detection membrane system is compressed between the first inner member and second inner member, wherein the conjugate pad is not compressed between the first and second inner members.
First outer member with removable or movable layer exposing the conjugate pad
The device includes a first outer member and the first outer member comprises a removable or movable layer that exposes the conjugate pad.
Outer members forming a container
The device is configured so that the first outer member and second outer member together form a container.
Container with removable or movable layer
A device includes a container whose structure has a removable or movable layer.
Kit including the device and optional positive/negative controls, instruction, buffer container, and sample collector
A kit including the device along with one or more optional components selected from a positive control, a negative control, an instruction booklet, a buffer container, and a sample collector.
Glass fiber conjugate pad
The device wherein the conjugate pad is a glass fiber conjugate pad.
Across the claim set, the core coverage is directed to a vertical-flow antigen detection device that compresses a stacked test membrane and absorbent member between contacting inner members while maintaining the conjugate pad uncompressed. Dependent refinements specify removable or movable structural layers for exposing the conjugate pad and further broaden scope to container configurations, kits with optional controls and handling components, and a glass fiber conjugate pad material.
Stated Advantages
Enables vertical flow by compressing the stacked analyte detection membrane system while leaving the conjugate pad not compressed.
Documented Applications
Detection of food-borne pathogen antigens using an antigen-detection device configured for antigen detection membrane systems.
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