Sheath fluid systems and methods for particle analysis in blood samples
Inventors
Farrell, Gregory A. • Wanders, Bart J. • Adams, Thomas H. • Groner, Warren • Zhao, Xiaodong
Assignees
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Publication Number
US-10451612-B2
Publication Date
2019-10-22
Expiration Date
Abstract
Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.
Core Innovation
The invention provides a particle and intracellular organelle alignment liquid (PIOAL) for use in a combined viscosity and geometric hydrofocusing analyzer. A blood sample fluid stream is injected into a narrowing flowcell transition zone of a visual analyzer and is enveloped by the PIOAL so that particles are brought to an imaging site in a target imaging state at an imaging site of the visual analyzer.
The PIOAL comprises a pH adjusting agent and a viscosity agent comprising glycerol at a concentration between about 1 and about 50% (v/v) and polyvinylpyrrolidone (PVP). The viscosity hydrofocusing effect is induced by an interaction between the PIOAL and the blood sample fluid associated with a viscosity difference, in combination with a geometric hydrofocusing effect induced by an interaction between the PIOAL and the blood sample fluid associated with the narrowing flowcell transition zone.
The viscosity agent retains viability of cells and leaves structure and content of the cells intact when the cells extend from the sample fluid stream into the PIOAL. In this way, the analyzer produces an optical imaging state at a focal plane while preserving cell viability and cell morphology, including intracellular organelle structures extending from cells into the PIOAL.
Claims Coverage
The partial document identifies one independent claim. Its coverage centers on a PIOAL composition and its use in a combined viscosity and geometric hydrofocusing visual analyzer, producing a target imaging state while retaining viability and preserving cell structure and content.
PIOAL for combined viscosity and geometric hydrofocusing analyzer
A particle and intracellular organelle alignment liquid (PIOAL) for use in a combined viscosity and geometric hydrofocusing analyzer, where a blood sample fluid is injected into a narrowing flowcell transition zone of a visual analyzer and is enveloped by the PIOAL to produce a sample fluid stream at an imaging site.
Isotonic aqueous PIOAL with defined viscosity
The PIOAL is a fluid with a viscosity between about 1 and about 10 centipoise, where the PIOAL is isotonic and the PIOAL is an aqueous solution.
pH adjusting agent and glycerol/PVP viscosity agent
The PIOAL comprises a pH adjusting agent and a viscosity agent comprising glycerol at a concentration between about 1 to about 50% (v/v) and polyvinylpyrrolidone (PVP).
Combined viscosity and geometric hydrofocusing to reach target imaging state
A viscosity hydrofocusing effect induced by an interaction between the PIOAL and the blood sample fluid associated with the viscosity difference, combined with a geometric hydrofocusing effect induced by an interaction between the PIOAL and the blood sample fluid associated with the narrowing flowcell transition zone, is effective to provide a target imaging state in at least some of a plurality of particles at an imaging site of the visual analyzer.
Viability retention and intact structure/content
The viscosity agent in the PIOAL retains viability of cells in the sample fluid stream and leaves structure and content of the cells intact when the cells extend from the sample fluid stream into the PIOAL.
Coverage is directed to an isotonic aqueous PIOAL with pH adjusting agent and a glycerol/PVP viscosity agent, used to envelop a blood sample stream in a narrowing flowcell transition zone so that combined viscosity and geometric hydrofocusing yields a target imaging state at an imaging site while retaining cell viability and preserving cell structure and intracellular content.
Stated Advantages
Provides a target imaging state in at least some of a plurality of particles at an imaging site of the visual analyzer.
Retains viability of cells in the sample fluid stream.
Leaves structure and content of the cells intact when cells extend from the sample fluid stream into the PIOAL.
Documented Applications
Use in a combined viscosity and geometric hydrofocusing visual analyzer for image-based blood particle analysis, including a visual analyzer with a narrowing flowcell transition zone.
WBC differential counting and morphological abnormality detection.
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