Magnetic particle separating device, and method of separating and purifying nucleic acid or protein using same
Inventors
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Assignees
Member
BioneerBioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Bioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Publication Number
US-10441957-B2
Publication Date
2019-10-15
Expiration Date
Abstract
The present invention relates to a magnetic particle separating device, and a method of separating and purifying nucleic acid or protein using the same. The device comprises: induction magnets (100); an induction magnet fixing part (200) having induction magnet fixing holes (210) for fixing the induction magnets (100); and a body (300) in which entry holes (310) are formed into which tubes (T) are inserted. Thus, the application and removal of a magnetic field to and from the body is made very convenient, so that the device can be very advantageously used in the separation and purification of nucleic acid or protein.
Core Innovation
The invention relates to a magnet particle separating device that includes a plurality of induction magnets with magnetic fields for attracting magnetic particles. An induction magnet fixing part has induction magnet fixing holes into which the induction magnets are inserted for fixing the induction magnets, and the induction magnet fixing part is combined with a body that has entry holes formed downward from a top surface along a vertical height direction.
The entry holes, including a first entry hole and a second entry hole adjacent to each other, correspond to an array position of the induction magnets. A compartment wall compartments the first entry hole for receiving a first tube and the second entry hole for receiving a second tube, and plural induction magnets are arranged in the vertical direction along each entry hole.
The compartment wall includes an insertion hole formed along a longitudinal direction orthogonal to the height direction, and a single tube-fixing member positioned in the insertion hole and exposed to the first and second entry holes. The single tube-fixing member compresses an outer surface of each tube being inserted along the height direction to fix the first and second tube to the body; alternatively, the body includes a groove portion penetrating the compartment wall and a single tube-fixing member positioned in the groove portion to press the outer surface of each inserted tube and fix the tubes.
Claims Coverage
The partial content provides one independent claim directed to an induction-magnet-based magnet particle separating device, with dependent claims specifying structural refinements and a nucleic-acid/protein separation workflow concept in at least one dependent claim.
Induction magnets fixed in an induction magnet fixing part aligned to entry holes
A magnet particle separating device comprising a plurality of induction magnets with magnetic fields for attracting magnetic particles, and an induction magnet fixing part having induction magnet fixing holes into which the induction magnets are inserted for fixing the induction magnets.
Body with vertically formed adjacent entry holes corresponding to magnet array position
A body combined with the induction magnet fixing part, the body having first and second entry holes formed downward from a top surface along a vertical height direction for inserting tubes such that the entry holes correspond to an array position of the induction magnets, and a compartment wall compartmenting each entry hole for receiving a tube.
Vertical arrangement of plural induction magnets along each entry hole
Plural induction magnets of the plurality of induction magnets are arranged in the vertical direction along each entry hole.
Single tube-fixing member compressing or pressing tube outer surfaces through compartment-wall structure
The compartment wall comprises an insertion hole along a longitudinal direction orthogonal to the height direction, with a single tube-fixing member positioned in the insertion hole and configured to compress outer surfaces of first and second tubes being inserted to fix the tubes; or the body comprises a groove portion penetrating the compartment wall along a width direction orthogonal to the height direction and the longitudinal direction, with a single tube-fixing member positioned in the groove portion and configured to press outer surfaces of the first and second tubes to fix the tubes.
Across the independent claim, the main inventive aspects are an induction-magnet fixing part with induction magnet fixing holes, a body with adjacent entry holes aligned to the induction-magnet array along a vertical direction, and a compartment-wall structure using a single tube-fixing member to fix tubes in place while magnetic fields attract magnetic particles.
Stated Advantages
Enables separating nucleic acid or protein using magnetic fields to attract magnetic particles carrying target gene material.
Documented Applications
Separating and purifying nucleic acid or protein by mixing gene material with magnetic particles in a tube and using magnetic fields to attract magnetic particles carrying the gene material.
Separating nucleic acid such as plasmid DNA, with electrophoresis showing similar DNA separation performance to a column-type plasmid kit.
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