Compositions and methods for disease diagnosis using single cell analysis
Inventors
Assignees
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Publication Number
US-10401349-B2
Publication Date
2019-09-03
Expiration Date
Abstract
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
Core Innovation
The disclosure describes single-cell diagnosis and characterization using a viability reporter and time-dependent optical waveforms associated with small-volume partitions. [procedural detail omitted for safety] The cell is identified through analysis of its waveform, with comparison to stored waveforms described as one identification approach. The partial content does not include the background section, so the problem being solved is not explicitly described in patent.
The disclosure also describes using waveform comparisons to support phenotypic antimicrobial susceptibility assessment. It reports distinguishable waveforms for bacterial species and describes improved drug-response discrimination from waveform analysis compared with endpoint measurement. These applications and findings are stated in the supplied summary; further details from the abstract, summary, and background sections are not included in the partial content.
Claims Coverage
The supplied claims identify one independent claim presenting one inventive feature directed to single-cell identification through waveform analysis.
Cell identification using an optical waveform
Claim 1 covers identifying a cell using information obtained by analyzing the optical waveform associated with its small-volume partition. [procedural detail omitted for safety]
The independent claim centers on identifying a cell through analysis of its optical waveform. The supplied claim information does not identify any other independent claims.
Stated Advantages
The disclosure states that small-volume confinement enables rapid, sensitive detection.
The disclosure states that multiplex identification is possible with a universal reporter.
The disclosure states that antimicrobial susceptibility results can be obtained in under 4–6 hours without cell lysis or washing.
The disclosure reports improved drug-response discrimination using waveform analysis compared with endpoint measurement.
Documented Applications
Identification and characterization of disease-causing cells, including bacterial species such as E. coli, Staphylococcus epidermidis, Bacillus subtilis, and Stenotrophomonas maltophilia.
Phenotypic antimicrobial susceptibility assessment using comparisons of cell-associated waveforms.
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