Multi-well cuvette provided with integrated reaction and detection means
Inventors
Kim, Byeong-Chul • MOON, Bong-Sok • Lee, Won-Hee • JEON, Chan-Young
Assignees
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Publication Number
US-10393738-B2
Publication Date
2019-08-27
Expiration Date
Abstract
The present application discloses a multi-well cuvette for integrating the reaction between a sample and a reagent with the detection of a specific analyte included therein, and the multi-well cuvette comprises: a reaction chamber part having a sample and a reagent placed therein; and a detection part for detecting the reaction between the sample and the reagent, wherein the reaction chamber part comprises: an extraction member standby chamber in which a member for dividing or distributing the sample is on standby; a sample filling chamber; and a reagent filling chamber which is filled with the reagent, and in which the reaction with the sample is carried out, wherein the detection part comprises: a chromatography analysis means for a reactive product between the sample and the reagent; and a detection chamber for accommodating the same.
Core Innovation
The invention relates to a disposable multi-well cuvette that integrates sequential reaction and analysis in a single cuvette body for biological/analyte testing. The cuvette includes a reaction part and an analysis part arranged in relation to a chromatographic strip so that a liquid reaction product is applied and then moved along the strip for later flow assay. The reaction part comprises a sample chamber configured to accommodate a liquid sample and a reagent chamber configured to accommodate a reagent for reaction with the liquid sample therein.
The analysis part comprises a reaction product well configured to hold a liquid reaction product and a chromatographic strip extending between a first strip end and a second strip end. The reaction product well and the chromatographic strip are arranged so that the sample chamber, the reagent chamber, and the reaction product well are aligned in a direction along extension of the chromatographic strip from the first strip end toward the second strip end.
The jig, the sample chamber, the reagent chamber, the reaction product well, and the chromatographic strip are integrated in the cuvette body. The cuvette is configured to permit the liquid reaction product, when applied to the reaction product well, to flow to the first strip end for a later flow assay in which the liquid reaction product further flows in the chromatographic strip in the direction from the first strip end toward the second strip end.
The document further describes an integrated detection architecture in which the analysis part extends from the reaction part and includes a lateral-flow chromatography cartridge in a detection chamber. The cartridge includes a strip receiving portion and a sample receiving well with a sample inlet and a measurement window, and capillary-action structures transfer the reaction product to the lateral-flow strip. Optionally, the cuvette can include a magnetic separation member beneath the sample receiving well to remove unwanted components using antibody-coated magnetic beads.
Claims Coverage
Independent claim clm-00001 defines a cuvette architecture integrating a jig, a reaction part (sample chamber and reagent chamber), and an analysis part (reaction product well and chromatographic strip) in a single cuvette body with a specific alignment along the strip direction and a defined flow path for a liquid reaction product. The inventive features are primarily the integrated alignment and fluid flow arrangement enabling a later flow assay along the chromatographic strip.
Integrated cuvette body with jig and aligned reaction-to-strip structure
The jig, the sample chamber, the reagent chamber, the reaction product well, and the chromatographic strip are integrated in a cuvette body; the sample chamber, the reagent chamber and the reaction product well are arranged between the jig and the first strip end such that they are aligned in a direction along extension of the chromatographic strip from the first strip end toward the second strip end.
Reaction product flow to first strip end for later flow assay
The cuvette is configured to permit the liquid reaction product, when applied to the reaction product well, to flow to the first strip end of the chromatographic strip for a later flow assay in which the liquid reaction product further flows in the chromatographic strip in the direction from the first strip end toward the second strip end.
Across the independent claim coverage provided, the core claimed inventive concept is the integrated cuvette body in which the sample chamber, reagent chamber, and reaction product well are aligned along the chromatographic strip direction, and the liquid reaction product is permitted to flow to the first strip end to enable later flow assay along the strip toward the second strip end.
Stated Advantages
Enables a later flow assay in the chromatographic strip by permitting the liquid reaction product to flow from the reaction product well to the first strip end and further along the strip toward the second strip end.
Documented Applications
Biological sample and analyte testing using a disposable cuvette with reaction and chromatographic lateral-flow detection architecture.
Use with assays for analytes including hsCRP, HbA1c, microalbumin, PSA, AFP, and cTnI as mentioned in the document.
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