Systems and methods for growing and harvesting cells
Inventors
Kasuto, Harel • Karnieli, Ohad • Hoffman, Dotan • Raviv, Lior
Assignees
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Publication Number
US-10392593-B2
Publication Date
2019-08-27
Expiration Date
Abstract
This disclosure is directed to methods and systems for growing and/or harvesting cells. The method can include seeding cells on a plurality of carriers. The method can also include incubating the carriers under conditions suitable for cell growth.
Core Innovation
The invention relates to a bioreactor system for growing and harvesting cells. The bioreactor includes a vessel having a first compartment and a second compartment positionable below the first compartment, where the second compartment is defined between a first perforated structure and a second perforated structure disposed above the first perforated structure, and the second perforated structure is moveably coupled to the vessel.
A rod is coupled to the second perforated structure, extending upward through a port in an upper plate of the vessel, and a locking element engages the upper plate and the rod to lock the second perforated structure in position in the vessel. The bioreactor is configured to seed cells in the first compartment while a plurality of carriers are disposed in the second compartment.
Each carrier includes a body with one or more two-dimensional (2D) surfaces extending inwardly from a periphery of the body towards an interior of the body. The bioreactor is configured to incubate the cells together with the carriers in the bioreactor under conditions compatible with growth and survival of the cells.
Claims Coverage
The partial content provides one independent claim. Across the provided claim set, the main inventive features are centered on the two-compartment vessel with moveable and lockable perforated structures, a rod passing through an upper plate, carriers having inward-extending 2D surfaces, and configurations for seeding cells in the first compartment while incubating with carriers in the second compartment.
Bioreactor with two-compartment vessel defined by perforated structures
A vessel comprising a first compartment and a second compartment positionable below the first compartment, the second compartment defined between a first perforated structure and a second perforated structure disposed above the first perforated structure, wherein the second perforated structure is moveably coupled to the vessel.
Rod and locking element for moveable second perforated structure
A rod coupled to the second perforated structure, the rod extending upward from the second perforated structure and through a port in an upper plate of the vessel, and a locking element that engages the upper plate and the rod and locks the second perforated structure in position in the vessel.
Carriers with inward-extending two-dimensional surfaces
Each carrier comprises a body, the body comprising one or more two-dimensional (2D) surfaces extending inwardly from a periphery of the body towards an interior of the body.
Seeding in first compartment while carriers are disposed in second compartment and incubation under cell-compatible conditions
The bioreactor configured to seed cells in the first compartment while the plurality of carriers are disposed in the second compartment, and configured to incubate the cells together with the carriers in the bioreactor under conditions compatible with growth and survival of the cells.
Overall claim coverage focuses on a bioreactor structure that physically and functionally separates seeding in a first compartment from carrier-held incubation in a second compartment defined by perforated structures, with mechanical locking via a rod and locking element and carriers characterized by inwardly extending 2D surfaces.
Stated Advantages
Documented Applications
No documented applications found
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