Virus like particle comprising modified envelope protein E3
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Publication Number
US-10385101-B2
Publication Date
2019-08-20
Expiration Date
Abstract
A virus like particle comprising a viral structural protein which comprises modified envelope protein E3. The viral structural protein may be that derived from or alphavirus or flavivirus. Especially, the viral structural protein may be derived from Chikungunya virus or Venezuelan equine encephalitis virus.
Core Innovation
The invention relates to virus-like particles comprising viral structural proteins that include a modified envelope protein E3. The virus-like particle comprises at least one foreign antigen inserted into the envelope protein E3, and the disclosure includes embodiments in which the inserted foreign antigen is introduced into other envelope components in addition to E3.
In additional embodiments, the envelope protein E3 is modified at a furin cleavage site by replacing, adding, and/or deleting one or more amino acid residues. The disclosure includes embodiments in which the furin cleavage site in E3 is altered or mutated so that cleavage does not occur, and these embodiments are directed to E3 processing and maturation behavior associated with protease handling and formation of the virus-like particle.
The disclosure further specifies examples of foreign antigens inserted into E3, including malaria Plasmodium falciparum circumsporozoite protein (NPNA repeats) and checkpoint or blockade antigens including PD-1, PD-L1, CTLA-4, DISC1, IL-2, HER2, BTLA, and HVEM. It also describes engineered antigen insertion formats and specific peptide options, and includes nucleic-acid and vector encodings for producing the virus-like particle, as well as compositions and kits comprising pharmaceutical or vaccine formulations.
Claims Coverage
The provided independent claim set contains 2 independent claims, which share the common theme of a virus-like particle having a modified envelope protein E3.
Foreign antigen insertion into modified envelope protein E3
A virus like particle comprising a viral structural protein which comprises a modified envelope protein E3, wherein at least one foreign antigen is inserted into said envelope protein E3.
Furin cleavage site amino-acid modification in envelope protein E3
A virus like particle comprising a viral structural protein which comprises a modified envelope protein E3, wherein one or more amino acid residues are replaced, added and/or deleted at a furin cleavage site in the envelope protein E3.
Overall, the claim coverage centers on virus-like particles whose envelope protein E3 is engineered either by inserting foreign antigens into E3 or by modifying amino-acid residues at the E3 furin cleavage site.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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