Regenerative cell and adipose-derived stem cell processing system and method
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Publication Number
US-10329533-B2
Publication Date
2019-06-25
Expiration Date
Abstract
An adipose-derived stem cell (ASC), regenerative cell and/or regenerative factor processing system including a tissue extraction device for extracting raw tissue, such as adipose tissue, from a patient, an ASC, regenerative cell and/or regenerative factor isolator, and an implantation device for re-introducing the isolated ASCs, regenerative cells and/or regenerative factors into the patient.
Core Innovation
The described invention relates to an adipose-derived stem cell processing device and related regenerative cell processing system for processing raw tissue to obtain adipose-derived stem cells. The system includes a tissue extraction device having chambers used for receiving and containing raw tissue and for processing tissue using sub-cavitation ultrasonic energy.
A first chamber receives raw tissue and an ultrasonic generator excites the raw tissue at sub-cavitation energy-levels and at the resonant frequency of cells, cellular structures, cell clusters, collagen fibrils and/or extracellular matrix components to induce liquefaction. A second chamber is fluidly-coupled to the first chamber via a valve, and a filter is configured between the first chamber and the second chamber to support isolation and separation between the chambers.
The processing system is further described as aiming to avoid enzymatic digestion in order to reduce immunologic reactions and tissue and extracellular matrix damage, thereby improving yield and reproducibility. The system can include filtration and separation using multi-chamber isolators, and can include additional enhancement steps such as osmotic pressure changes and mechanical disruption, with optional re-implantation and cryopreservation.
Claims Coverage
The provided content identifies one independent claim for a device and dependent claims that refine device structures and process parameters. The inventive features focus on sub-cavitation ultrasonic excitation at resonant frequency, chamber coupling with a valve, and filtration between chambers, with further refinements for tissue handling and ultrasound parameter cycling.
Adipose-derived stem cell processing device with first and second chambers and interposed filter
An adipose-derived stem cell processing device having a first chamber for receiving and containing raw tissue, a second chamber fluidly-coupled to the first chamber via a valve, and a filter configured between the first chamber and the second chamber.
Sub-cavitation ultrasonic liquefaction at resonant frequency of cells and extracellular structures
An ultrasonic generator configured with the first chamber to induce liquefaction of the raw tissue by exciting the raw tissue at sub-cavitation energy-levels and at the resonant frequency of cells, cellular structures, cell clusters, collagen fibrils and/or extracellular matrix components.
Adipose tissue as the raw tissue
The device where the raw tissue includes adipose tissue.
Receiving raw tissue using negative pressure
The device/system receiving raw tissue using negative pressure supplied by a vacuum source.
Ultrasonic generator cycling based on size of cellular and extracellular structures
The device/system in which the ultrasonic generator cycles through different durations, frequencies, and amplitudes based on the size of cells, cellular structures, cell clusters, collagen fibrils, and/or extracellular matrix components.
Overall, the claim coverage centers on a chamber-based adipose-derived stem cell processing device that uses an ultrasonic generator to induce liquefaction at sub-cavitation energy levels and resonant frequency of cells and extracellular structures, while using a valve-coupled second chamber and a filter between chambers. The dependent features further specify adipose tissue, negative-pressure tissue receipt, and ultrasound parameter cycling linked to the size of cellular and extracellular structures.
Stated Advantages
Avoids enzymatic digestion to reduce immunologic reactions and tissue/extracellular matrix damage.
Improves yield and reproducibility.
Enables closed aseptic processing.
Documented Applications
Processing raw tissue to obtain adipose-derived stem cells/regenerative cells for regenerative use, including optional re-implantation and cryopreservation.
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