Benchtop nucleic acid library preparation device and methods for using the same
Inventors
Barrett, Brian • Sirr, Noel • Chawke, Brian • Curran, Kieran • Daly, John
Assignees
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Publication Number
US-10207271-B2
Publication Date
2019-02-19
Expiration Date
Abstract
Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple CLC reaction wells; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each CLC reaction well of the thermal chip module.
Core Innovation
The invention provides a complete bench-top nucleic acid library preparation device that generates next generation sequencing (NGS) libraries using composite liquid cell (CLC) technology. The device includes a thermal chip module configured to receive a CLC reaction cartridge, a consumable reagent location for receiving a CLC nucleic acid library preparation reagent cartridge, and a sample location configured to receive a CLC sample cartridge, with the thermal chip module, the consumable reagent location, and the sample location positioned on a main deck of the device.
A robotically controlled liquid handler transfers liquid between the consumable reagent location, the sample location, and the thermal chip module. At least one well of the CLC reaction cartridge, the CLC nucleic acid library preparation reagent cartridge, or the CLC sample cartridge comprises a carrier fluid and an encapsulating fluid.
This CLC well arrangement supports the generation of barcoded/pooled nucleic acid libraries for next-generation sequencing, and CLC well design concepts include carrier fluid, encapsulating fluid, and target/sample fluid.
Claims Coverage
The independent claim covers 2 inventive features centered on a complete bench-top CLC nucleic acid library preparation device and CLC wells comprising carrier fluid and encapsulating fluid.
Complete bench-top CLC nucleic acid library preparation device on a main deck
A complete bench-top nucleic acid library preparation device comprising a thermal chip module configured to receive a composite liquid cell (CLC) reaction cartridge; a CLC reaction cartridge positioned at the thermal chip module and comprising a plurality of wells; a consumable reagent location configured to receive a CLC nucleic acid library preparation reagent cartridge; a CLC nucleic acid library preparation reagent cartridge positioned at the consumable reagent location and comprising a plurality of wells; a sample location configured to receive a CLC sample cartridge; a CLC sample cartridge positioned at the sample location and comprising a plurality of wells; and a robotically controlled liquid handler configured to transfer liquid between the consumable reagent location, the sample location, and the thermal chip module, wherein the thermal chip module, the consumable reagent location, and the sample location are positioned on a main deck of the device.
CLC wells comprising carrier fluid and encapsulating fluid
At least one well of the CLC reaction cartridge, the CLC nucleic acid library preparation reagent cartridge, or the CLC sample cartridge comprises a carrier fluid and an encapsulating fluid.
Overall claim coverage centers on a bench-top CLC-based nucleic acid library preparation device with a thermal chip module and cartridge well structure positioned on a main deck, robotically controlled liquid transfer, and at least one well containing both carrier fluid and encapsulating fluid.
Stated Advantages
Documented Applications
No documented applications found
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