Biocoated piezoelectric biosensor platform for point-of-care diagnostic use

Inventors

Laury-Kleintop, Lisa • Rutner, Herman

Assignees

AVIANA MELECULAR TECHNOLOGIES LLC • Aviana Molecular Technologies LLC

Abstract

Biosensor components (chips) are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezo-electrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays. The table mono layers of the anchor substances forms reactive layers, ready to bind a capture reagent such as a biot-inylated antibody for capture and detection of analytes in biologic fluid samples. Although the processes described herein can be performed on any type of piezoelectric material in any number of configurations, some embodiments are directed to a biosensor with the foregoing biocoating onto a particular acoustic plate mode biosensor and where the interdigitated transducers (IDTs) are present on the opposite side of the crystal's biocoated film.

Core Innovation

The invention relates to an acoustic wave biosensor component in which a piezoelectric crystal includes a layer of an anchor substance that is directly and irreversibly bound chemisorptively as a monolayer to the crystal surface without an intermediary coating. The anchor substance has the property of binding to a capture reagent comprising a specific binding partner for the anchor substance, providing a specific binding interface for downstream recognition.

An acoustic wave biosensor is further provided with the chemisorbed anchor layer coupled to capture reagent functionality so that the capture reagent specifically recognizes an analyte present in a biological fluid. The biosensor includes an acoustic wave generator that generates a wave in which a reaction between the capture reagent and an analyte causes a detectable change in properties of the acoustic wave. A chamber is provided for receiving a biological fluid sample.

A process is described for coating the surface of a piezoelectric material with a biofilm anchor substance by treating a crystal surface with plasma treatment to increase surface energy, applying the anchor substance directly and irreversibly without an intermediary coating, and forming a chemisorbed anchor layer as a monolayer. The coated surface supports binding to a capture reagent for specific recognition of analytes in biological fluid samples.

Claims Coverage

The partial content includes three independent claims. The claims center on a direct chemisorbed monolayer anchor on a piezoelectric crystal, the plasma-assisted coating process to form that monolayer, and an acoustic wave biosensor architecture that detects a capture reagent/analyte reaction in a biological fluid sample.

Across the independent claims, the coverage is directed to forming a direct, irreversible chemisorptive anchor monolayer on a piezoelectric crystal surface without intermediary coating, linking that anchor to a capture reagent that specifically recognizes an analyte in a biological fluid, and detecting the resulting reaction as a detectable change in properties of an acoustic wave, with a chamber for sample reception.

Stated Advantages

Documented Applications