Sample test method, microfluidic device, and test device
Inventors
Park, Sung Ha • PARK, Sang Bum • Lee, Beom Seok • Kim, Kui Hyun • PARK, Joo Hee • SONG, Kyung Mi • CHO, Euy Hyun • Kim, Ha Na
Assignees
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Publication Number
US-10126232-B2
Publication Date
2018-11-13
Expiration Date
Abstract
A sample test method, microfluidic device, and test device efficiently and accurately compensates for interference of an interfering substance present in a sample using optical measurement without addition of a separate reagent for detecting the interfering substance. The sample test method includes: measuring an optical characteristic value of a target substance present in a sample; measuring an optical characteristic value of an interfering substance present in the sample; and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
Core Innovation
The described sample test method is an in vitro diagnostic test that determines a concentration of a target substance while compensating for interference from an interfering substance. The method measures optical characteristic values for both the target substance and the interfering substance present in the same sample, and then determines an interference-compensated concentration of the target substance based on the optical characteristic value of the interfering substance. The determination uses optical characteristic values related to concentration, including effective optical density (OD_F) and effective concentration (C_Eff).
Interference compensation is performed by determining the concentration of the target substance based on the optical characteristic value of the target substance, determining the concentration of the interfering substance based on the optical characteristic value of the interfering substance, and compensating the target concentration using the interfering concentration. In particular embodiments, a fluctuation coefficient is used to compute an application result from the interfering substance concentration, and the application result is subtracted from or added to the target concentration to compensate interference.
The disclosed optical characteristic evaluation includes measurement and processing using main/sub-wavelength subtraction and/or multi-wavelength differences. Main-sub wavelength subtraction is performed by measuring optical characteristic values at a main wavelength and a sub-wavelength and using subtraction, and optional wavelength correction schemes are described using additional wavelength pairs. Multi-band processing is described using differences across wavelength bands to derive optical characteristic values relevant to the interfering substance.
Claims Coverage
The partial document contains two independent claims. Across these claims, there are four inventive features focused on optical measurement of both target and interfering substances, deriving concentrations from optical characteristic values, and compensating the target concentration based on the interfering substance concentration.
Optical measurement of target and interfering substances for interference compensation
A sample test method measuring an optical characteristic value of a target substance present in a sample and measuring an optical characteristic value of an interfering substance present in the sample, and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
Concentration determination from optical characteristic values and compensation using interfering concentration
A sample test method where determining the concentration of the target substance for which interference is compensated comprises determining the concentration of the target substance based on the optical characteristic value of the target substance, determining the concentration of the interfering substance based on the optical characteristic value of the interfering substance, and compensating the concentration of the target substance using the concentration of the interfering substance.
Optical measurement in a test device with processor-based interference compensation
A test device comprising a measurer configured to measure an optical characteristic value of a target substance present in a sample and an optical characteristic value of an interfering substance present in the sample, and a data processor configured to determine a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
Processor determines target/interfering concentrations and compensates target concentration
A test device where the data processor is configured to determine the concentration of the target substance based on the optical characteristic value of the target substance, determine the concentration of the interfering substance based on the optical characteristic value of the interfering substance, and compensate the concentration of the target substance using the concentration of the interfering substance.
Both independent claims revolve around measuring optical characteristic values of a target substance and an interfering substance in the same sample, determining concentrations from those optical values, and compensating the target concentration using the interfering substance concentration.
Stated Advantages
Improved correlation/accuracy after interference compensation as described in the experimental results.
Reduction of interference effects as described in the experimental results.
Supports improved accuracy across concentration ranges using chamber/light-path designs based on linearity.
Documented Applications
In vitro diagnostic sample testing for determining a target analyte concentration (example given: gamma-glutamyl transferase (GGT) in blood) while compensating interference (example given: bilirubin).
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