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Publication Number
US-10107727-B2
Publication Date
2018-10-23
Expiration Date
Abstract
A method including placing a biological sample taken from a body into a chamber; adding a composition including an acetal solvent to the chamber; and fixating the biological sample. A method including placing a tissue from a body into a chamber; and contacting the tissue with a composition including an acetal solvent as a fixating process.
Core Innovation
The disclosed approach provides tissue processing of a biological sample taken from a body by placing the biological sample into a chamber and adding a composition comprising an acetal solvent. The biological sample is fixated, and after fixating the biological sample, it is processed by being contained with a paraffin. The document further describes embodiments in which the acetal solvent is used as part of a composition added to the chamber as a component of the fixating and/or subsequent tissue processing workflow.
The document focuses on using acetals, including dioxolane and 1,3-dioxolane, as acetal solvents to treat the biological sample through pre-analytical tissue processing stages. In disclosed workflows, acetal solvent treatment is linked to dehydration, clearing, and infiltration steps that occur after fixating. The described processing includes integrating acetal solvent treatment with embedding, including paraffin infiltration and embedding with paraffin wax.
The document also describes an in-situ hydrolysis concept in which dioxolane can generate formaldehyde for fixation while the acetal remains available to aid clearing and/or dehydration. The in-situ hydrolysis concept is described together with the use of catalysts, including acid or Lewis acid catalysts, and with water in the acetal-containing composition. The overall method is presented as a chamber-based process that coordinates fixating and subsequent pre-analytical processing using an acetal-solvent composition.
Claims Coverage
The partial content provides two independent claims for acetal-solvent based tissue processing, with dependent claims adding specific acetal identity, optional catalyst and water in the acetal-containing composition, and additional post-fixation processing steps such as dehydrating, clearing, and infiltration with embedding agent or paraffin.
Acetal-solvent added fixation with paraffin containment after fixating
Placing a biological sample taken from a body into a chamber; adding a composition comprising an acetal solvent to the chamber; fixating the biological sample; and after fixating the biological sample, containing the biological sample with a paraffin.
Acetal solvent as a fixating process followed by infiltration with embedding agent
Placing a tissue taken from a body into a chamber; contacting the tissue with a composition comprising an acetal solvent as a fixating process; and after the fixating process, infiltrating the tissue with an embedding agent in an infiltration process.
Across the independent claims, the core claim coverage centers on chamber-based tissue processing that uses an acetal-solvent composition to perform fixation, followed by embedding-related infiltration using paraffin or an embedding agent, with dependent claim refinements specifying dioxolane or 1,3-dioxolane and optional inclusion of water and catalysts such as acid or Lewis acid.
Stated Advantages
Documented Applications
No documented applications found
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