Red transgenic fluorescent ornamental fish
Inventors
Blake, Alan • Crockett, Richard • Nasevicius, Aidas
Assignees
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Publication Number
US-10098334-B2
Publication Date
2018-10-16
Expiration Date
Abstract
The present invention relates to transgenic red ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Core Innovation
The invention relates to transgenic red fluorescent ornamental fish, including a transgenic albino barb (Puntius tetrazona) comprising a chromosomally integrated red fluorescent protein expression cassette and exhibiting fluorescence. The expression cassette is comprised by the Red barb 1 transformation event, and sperm comprising the Red barb 1 transformation event has been deposited as ECACC accession no. 16121901.
The invention further provides configurations of transgenic and breeding-related populations in which progeny can be produced carrying the Red barb 1 transformation event. It encompasses progeny and transgenic offspring derived from breeding, including transmission states referenced in the partial content such as homozygous, heterozygous, and hemizygous for the transformation event.
Beyond the fish themselves, the partial content describes providing transgenic fish to the ornamental fish market and distributing the transgenic fish through market roles, including grower, commercial distributor, and retailer. The partial content also describes use cases as scientific research tools, including cell lineage and migration, mosaic labeling, genetic mosaic marking, and fish cancer models, where visualization by red fluorescence is used.
Claims Coverage
The independent claims define 2 main inventive feature groupings: the specific Red barb 1 transformation event-defined transgenic barb and the breeding-based production method maintaining that event with fluorescence in progeny.
Chromosomally integrated Red barb 1 event encoding red fluorescent protein exhibiting fluorescence
A transgenic barb whose genome comprises a chromosomally integrated expression cassette encoding a red fluorescent protein, the expression cassette being comprised by the Red barb 1 transformation event, where sperm comprising the Red barb 1 transformation event has been deposited as ECACC accession no. 16121901, and wherein the barb exhibits fluorescence.
Producing transgenic barb by obtaining Red barb 1 event fluorescent barb and breeding with a second barb
A method of producing a transgenic barb comprising obtaining a barb that exhibits fluorescence and comprises a chromosomally integrated expression cassette encoding a red fluorescent protein, wherein said expression cassette is comprised by the Red barb 1 transformation event, sperm comprising the Red barb 1 transformation event having been deposited as ECACC accession no. 16121901; and breeding the obtained barb with a second barb to provide a transgenic barb comprising the Red barb 1 transformation event, wherein the transgenic barb exhibits fluorescence.
Overall, the claim coverage centers on the Red barb 1 transformation event (ECACC accession no. 16121901) as the defining source of fluorescence via a chromosomally integrated red fluorescent protein expression cassette, and on breeding-based methods that yield additional transgenic barbs comprising that same event and exhibiting fluorescence.
Stated Advantages
Provides transgenic barb exhibiting fluorescence attributable to the Red barb 1 transformation event with a chromosomally integrated red fluorescent protein expression cassette.
Enables production of transgenic barbs comprising the Red barb 1 transformation event with fluorescence for downstream ornamental fish market distribution.
Documented Applications
Visualization and use as scientific research tools, including cell lineage and migration, mosaic labeling, genetic mosaic marking, and fish cancer models, using red fluorescence.
Providing transgenic fish to the ornamental fish market, including distribution through grower, commercial distributor, and retailer.
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