Method of manufacturing micro chamber plate with built-in sample and analytic micro chamber plate, analytic micro chamber plate and apparatus set for manufacturing analytic micro chamber plate with built-in sample
Inventors
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Assignees
Member
BioneerBioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Bioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Publication Number
US-10022717-B2
Publication Date
2018-07-17
Expiration Date
Abstract
A method of manufacturing a micro-chamber plate with a built-in sample, including: settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening; disposing a cover for micro-chamber plate receiving part to cover the upper opening, the cover for micro-chamber plate receiving part having a provisional storing part and an auxiliary covering part connected with the provisional storing part and formed with a through-hole for auxiliary covering part; and manufacturing a micro-chamber plate with a built-in sample by putting the micro-chamber plate receiving part, on which the cover is disposed, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate through a vessel communication part which is formed at the provisional storing part to be communicated with the micro-chamber plate receiving part.
Core Innovation
The invention relates to a method of manufacturing a micro-chamber plate with a built-in sample by using a cover that includes a provisional storing part with a cutting line and an auxiliary covering part with a through-hole. The cover is disposed to cover an upper opening of a micro-chamber plate receiving part, so that the cutting line is communicated with the micro-chamber plate receiving part. A sample solution provisionally stored in the provisional storing part is injected into the micro-chamber plate for sample injection through the cutting line.
The method uses a centrifugal separator capable of applying centrifugal force and vacuum to perform the injection. The micro-chamber plate, on which the cover is disposed, is placed in the centrifugal separator, and the provisionally stored sample solution is injected through the cutting line to communicate with the micro-chamber plate receiving part. A vacuum-and-centrifugal-force sequence suppresses bumping when the cutting line is closed.
In an additional aspect, an analytic micro-chamber plate is manufactured by sealing a separation membrane of the micro-chamber plate with the built-in sample after the centrifugal processing. The document also describes forming a sample solution storing space by closely contacting the cover with the micro-chamber plate, and then injecting the provisionally stored sample solution through the cutting line into the micro-chamber plate for sample injection.
Claims Coverage
The document provides two independent claims describing the manufacturing of a micro-chamber plate with a built-in sample. The inventive coverage centers on a provisional storing part with a cutting line, vacuum-capable centrifugal injection, and an embodiment in which the cover and micro-chamber plate are closely contacted to form a sample solution storing space.
Provisional storing part with cutting line and auxiliary covering part with through-hole
A cover includes a provisional storing part formed with a cutting line and an auxiliary covering part connected with the provisional storing part and formed with a through-hole, the cover being disposed to cover the upper opening of a micro-chamber plate receiving part so the cutting line is formed for communication with the micro-chamber plate receiving part.
Centrifugal separator applying centrifugal force and vacuum
The micro-chamber plate with the cover disposed thereon is put into a centrifugal separator capable of applying centrifugal force and vacuum.
Injection of provisionally stored sample solution through cutting line
A sample solution provisionally stored in the provisional storing part is injected into the micro-chamber plate for sample injection through the cutting line formed at the provisional storing part to be communicated with the micro-chamber plate receiving part.
Closely contacting cover and micro-chamber plate to form sample solution storing space
A sample solution storing space is formed between the cover and an upper surface of the micro-chamber plate for sample injection by closely contacting a lower end of the cover to the upper surface.
Provisional sample solution injected to communicate with sample solution storing space
The micro-chamber plate for sample injection and the cover, which are closely contacted with each other to form the sample solution space therebetween, are put into the centrifugal separator, and a sample solution provisionally stored in the provisional storing part is injected into the micro-chamber plate for sample injection through the cutting line formed at the provisional storing part so as to be communicated with the sample solution storing space.
Across the independent claims, the inventive coverage centers on manufacturing a micro-chamber plate with a built-in sample using a cover having a provisional storing part with a cutting line and an auxiliary covering part with a through-hole, placing the assembly in a centrifugal separator that applies centrifugal force and vacuum, and injecting the provisionally stored sample solution through the cutting line to the micro-chamber plate for sample injection, including embodiments where a sample solution storing space is formed by closely contacting the cover and the micro-chamber plate.
Stated Advantages
Reduced evaporation.
Reduced cross contamination.
Suppression of bubbles and remained gas.
Improved fluorescence measurement accuracy.
Reduced analysis time.
Smaller plate size.
Reduced manufacturing cost via integral formation and compression molding.
Documented Applications
Manufacturing an analytic micro-chamber plate for use in analytic micro-chamber processing such as real-time PCR.
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