Allergen desensitization method
Inventors
Dupont, Christophe • Benhamou, Pierre-Henri • Dupont, Bertrand
Assignees
Assistance Publique Hopitaux de Paris APHP • DBV Technologies SA • Universite Paris Cite
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Publication Number
US-10022439-B2
Publication Date
2018-07-17
Expiration Date
Abstract
The present application relates to a method for desensitization of allergic patients. More specifically it relates to an epicutaneous desensitization method, applicable to any type of allergens and of patients. The method of the invention is essentially non-invasive and does not require the use of adjuvants. Further, it may be easily applied and monitored by the actual patient.
Core Innovation
The invention relates to a method for epicutaneous desensitization of a subject allergic to milk. The method determines a dose of a milk allergen that causes a local inflammatory reaction on the skin upon epicutaneous application, and the subject is epicutaneously exposed to the milk allergen to generate the local inflammatory reaction on an intact region of skin without an adjuvant.
The method repeatedly applies the milk allergen, without an adjuvant, to an intact region of the skin at a dose, frequency or application time adapted to sustain a local inflammatory reaction. The exposure and sustained reaction are used to lead to desensitization of the subject, and the milk allergen is applied by a cutaneous occlusive device configured to ensure contact between the allergen and the region of skin.
The dose of milk allergen is comprised between 0.1 and 1,000 μg/cm2 of a surface area of the device. The approach is based on controlling and sustaining a cutaneous inflammatory reaction to orient the immune response toward tolerance, and the local inflammatory reaction may be monitored by visual grading and, optionally, cytokine markers.
Claims Coverage
The independent claim covers a milk-allergen epicutaneous desensitization method defined by selecting a dose that induces a local inflammatory reaction without an adjuvant, repeatedly applying the allergen at parameters adapted to sustain that reaction, and using a cutaneous occlusive device to ensure skin contact, with the allergen dose constrained to a defined surface-area range.
Dose determination to induce local inflammatory reaction without an adjuvant
Determining a dose of a milk allergen which causes a local inflammatory reaction on the skin of the subject upon epicutaneous application thereof.
Adjuvant-free epicutaneous exposure on intact skin to generate the inflammatory reaction
Epicutaneously exposing the subject to the milk allergen and generating the local inflammatory reaction in the subject by applying said dose of the milk allergen, without an adjuvant, onto an intact region of skin of the subject.
Repeated adjuvant-free exposure with parameters adapted to sustain local inflammatory reaction leading to desensitization
Repeatedly applying the milk allergen, without an adjuvant, to an intact region of the skin of the subject, at a dose, frequency or for an application time adapted to sustain a local inflammatory reaction, the exposure and sustained reaction leading to desensitization of the subject.
Occlusive device ensuring contact between allergen and skin
The milk allergen is applied by a cutaneous occlusive device configured to ensure contact between the allergen and the region of skin.
Defined allergen dose per device surface area
The dose of milk allergen is comprised between 0.1 and 1,000 μg/cm2 of a surface area of the device.
Overall claim coverage centers on an adjuvant-free epicutaneous protocol for milk-allergic subjects in which a determined allergen dose triggers and the repeated application parameters sustain a local cutaneous inflammatory reaction, using a cutaneous occlusive device for skin contact and restricting the allergen dose to a specified μg/cm2 range.
Stated Advantages
Epicutaneous desensitization is performed without an adjuvant.
The sustained local inflammatory reaction is used to lead to desensitization of the subject.
Documented Applications
Epicutaneous desensitization of a subject allergic to milk.
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