Cell seeding device and method
Inventors
Wang, Yongzhong • Kelly, Christopher • RAPKO, Stephen • QUINNO, Frank • Seymour, Barbara
Assignees
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Publication Number
US-10011811-B2
Publication Date
2018-07-03
Expiration Date
Abstract
Described is a device comprising an anchor, a tray including a well adapted to receive the anchor, and a cover adapted to engage the tray and cover the well. The device may be used to form a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions.
Core Innovation
The invention is a cell-seeding device comprising an anchor, a tray with a well adapted to receive the anchor, and a cover adapted to engage the tray and cover the well. The anchor has a sidewall with an upper portion disposed approximately parallel to the longitudinal axis of the device and a lower portion angled radially toward that axis. The lower portion engages at least a portion of a membrane when the membrane is present.
The anchor and well confine cell-containing medium over the membrane to promote substantially uniform cell distribution in at least two dimensions. The disclosed method forms and removes a membrane-cell matrix. The partial content does not include the background section, so the problem being solved from that section is not explicitly described in patent.
Claims Coverage
The provided relevant claims identify one independent claim, claim 1, with three main inventive features.
Anchor with angled lower sidewall portion
The anchor comprises at least one sidewall with an upper portion disposed in a plane approximately parallel to the longitudinal axis of the device and a lower portion with an outer surface angled radially toward that axis, wherein the lower portion engages at least a portion of the membrane when present.
Tab disposed in the plane of the upper portion
At least one tab is disposed on the upper portion of at least one sidewall and in the same plane as the upper portion on which it is disposed.
Slidable anchor, tray well, and cover
The tray includes a well adapted to receive the anchor, the anchor is adapted to be slidably disposed in the well, and the cover is adapted to engage the tray and cover the well.
Claim 1 covers the anchor's sidewall and tab arrangement, the anchor's slidable placement in the tray well, and the cover's engagement with the tray and coverage of the well.
Stated Advantages
The anchor and well confine cell-containing medium over the membrane to promote substantially uniform cell distribution in at least two dimensions.
Documented Applications
The disclosed method generates a membrane-cell matrix with substantially uniform cell distribution in at least two dimensions.
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