SmartFreez


Smartfreez specializes in developing innovative cryoprocessing systems for the biopharma industry, utilizing advanced computational simulation tools to optimize storage stability of biologics. Their mission is to help researchers and manufacturers preserve sensitive biologic products at large scales, build and validate cryopreservation processes with minimal product, and accelerate time to market through process qualification and optimization.

Industries

biotechnology
consulting
developer-tools
life-science

Nr. of Employees

small (1-50)

SmartFreez

Av. Prof. Dr. Cavaco Silva, Ed. Tecnologia 1.2, nº 21, 2740-257 Porto Salvo – Portugal


Patents

Apparatus and method for measuring degradation kinetics of a biomaterial in aqueous solution below the normal freezing temperature

US-12352741-B2

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Insulator for preventing container damage and rupture caused by freezing of aqueous solutions containing biological materials

US-12336532-B2

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Device and method for freezing a biological solution

US-12302895-B2

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Differential air flow system for promoting bottom-up freezing of blood plasma in compressed bags

US-12268206-B2

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System for scale-down the processes of freezing and thawing aqueous solutions of thermo-sensitive pharmaceuticals

US-12262709-B2

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Products

Cold-stability accelerator instrument

Laboratory instrument that imposes isochoric/isothermal conditions to measure subzero degradation and cold denaturation without bulk freezing, enabling accelerated stability evaluation.

Micro scale-down freeze–thaw device

Low-volume physical model that reproduces stress-time distributions of large containers using controlled ice-nucleation and heat-flow in vials (<10 mL) for representative freeze–thaw experiments.

Cell cryopreservation instrument with directional heat control

Instrument designed with unidirectional heat-transfer geometry and controlled sacrificial supercooling layer to minimize thermal and mechanical stress on cells during freezing.

Single-use container thermal/pressure shield

Single-use insulating shield for individual bottles or bags providing thermal isolation, pressure mitigation during freezing, and physical protection during transport and handling.

Isothermal insulating cover for bottles

Reusable insulating cover for bottles and carboys that prevents ice-crust formation and reduces pressure development and protein aggregation during freezing.


Services

Provision of cloud-based CFD digital-twin simulations to predict freeze–thaw behaviour and guide method/equipment design.

Lab-based freeze–thaw experiments using low-volume scale-down devices to reproduce manufacturing freeze–thaw stress with minimal product consumption.

Measurement and modelling of protein cold aggregation and cryoprotectant effects to inform formulation strategies and predict long-term stability.

Design, customization and supply of scalable freeze–thaw equipment and single-use shields for freezing, transport, storage and thawing of biologics in bottles or bags.

Expertise Areas

  • Cryopreservation process development
  • CFD simulation of phase change in biological systems
  • Accelerated cold-stability testing and aggregation modelling
  • Low-volume scale-down modelling for freeze–thaw
  • Show More (3)

Key Technologies

  • Computational Fluid Dynamics (CFD)
  • Digital twin/cloud simulation
  • Accelerated isothermal cold-stability testing
  • Low-volume scale-down physical modelling
  • Show More (3)

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