highQu
Manufacturer and supplier of molecular biology reagents and kits for PCR/qPCR, one-step reverse transcription qPCR, isothermal amplification and supporting consumables. Capabilities include recombinant enzyme production (DNA polymerases including hot-start and high-fidelity, strand-displacement polymerases, reverse transcriptases, RNase inhibitors), formulation of concentrated master mixes (2X/4X, ROX variants), dNTP sets, electrophoresis stains and ladders, sample lysis solutions for direct PCR, lateral-flow detection for amplified products, and OEM/custom bulk manufacturing. Provides product inserts, MSDS and lot-specific Certificates of Analysis on request. Several kits are documented for low-copy viral RNA detection and for fast cycling and multiplex assays.
Industries
Nr. of Employees
small (1-50)
highQu
Ubstadt-weiher, Baden-Wurttemberg, Germany, Europe
Products
Concentrated one‑step RT‑qPCR master mixes (2X/4X; ROX variants)
Concentrated RT‑qPCR and qPCR master mixes combining buffer, dNTPs and hot‑start polymerase with thermostable RT and RNase inhibitor blends in formats suitable for fast cycling, multiplex assays and high sample input.
Hot‑start Taq polymerase mastermixes (ready‑to‑load dye variants)
Hot‑start Taq DNA polymerase formulations and 2X mastermixes including ready‑to‑load red dye/density reagents for direct gel loading and reduced pipetting steps; suitable for crude‑sample PCR and fast cycling.
High‑fidelity DNA polymerase and mastermixes for long amplicons
High‑fidelity polymerase formulations optimized for low error rate, increased processivity and higher yields on long or GC‑rich templates; available as enzymes and 2X mastermixes for cloning, sequencing and NGS library preparation.
Strand‑displacement polymerase and isothermal amplification kits
Glycerol‑free strand‑displacement polymerase formulations and isothermal master mixes for LAMP, RAM, WGA and related assays, with fluorescent or colorimetric readouts and readiness for lyophilized kit formats.
Colorimetric isothermal amplification master mixes
Isothermal amplification mixes incorporating pH‑sensitive colorimetric indicators to enable instrument‑free visual detection (color change) for point‑of‑care applications, formulated with hot‑start polymerase chemistry for ambient setup.
Thermostable reverse transcriptase mixes for long cDNA synthesis
Reverse transcriptase mixes formulated for efficient cDNA synthesis of long transcripts (reported up to 12–15 kb) with broad reaction temperature range and premixed buffers to reduce pipetting steps.
Concentrated one‑step RT‑qPCR master mixes (2X/4X; ROX variants)
Concentrated RT‑qPCR and qPCR master mixes combining buffer, dNTPs and hot‑start polymerase with thermostable RT and RNase inhibitor blends in formats suitable for fast cycling, multiplex assays and high sample input.
Hot‑start Taq polymerase mastermixes (ready‑to‑load dye variants)
Hot‑start Taq DNA polymerase formulations and 2X mastermixes including ready‑to‑load red dye/density reagents for direct gel loading and reduced pipetting steps; suitable for crude‑sample PCR and fast cycling.
High‑fidelity DNA polymerase and mastermixes for long amplicons
High‑fidelity polymerase formulations optimized for low error rate, increased processivity and higher yields on long or GC‑rich templates; available as enzymes and 2X mastermixes for cloning, sequencing and NGS library preparation.
Strand‑displacement polymerase and isothermal amplification kits
Glycerol‑free strand‑displacement polymerase formulations and isothermal master mixes for LAMP, RAM, WGA and related assays, with fluorescent or colorimetric readouts and readiness for lyophilized kit formats.
Colorimetric isothermal amplification master mixes
Isothermal amplification mixes incorporating pH‑sensitive colorimetric indicators to enable instrument‑free visual detection (color change) for point‑of‑care applications, formulated with hot‑start polymerase chemistry for ambient setup.
Thermostable reverse transcriptase mixes for long cDNA synthesis
Reverse transcriptase mixes formulated for efficient cDNA synthesis of long transcripts (reported up to 12–15 kb) with broad reaction temperature range and premixed buffers to reduce pipetting steps.
Services
OEM and custom reagent formulation
Contract formulation, optimization and bulk production of molecular biology reagents and kits with options for OEM labeling, custom concentrations and lot‑specific Certificates of Analysis.
Technical support, documentation and sample requests
Provision of protocols, product inserts, MSDS files, performance notes and publication references; options for requesting samples and technical application support.
Sample preparation and direct‑PCR workflow support
Supply of sample lysis buffers, protease reagents and validated direct‑PCR protocols for a range of clinical, environmental and research sample types, with guidance on inhibitor mitigation and compatibility with inhibitor‑resistant master mixes.
Lyophilized/dry kit formulation for field assays
Development support for glycerol‑free enzyme formulations and master mixes compatible with lyophilization to produce dry kit formats for point‑of‑care or ambient‑stable assays.
OEM and custom reagent formulation
Contract formulation, optimization and bulk production of molecular biology reagents and kits with options for OEM labeling, custom concentrations and lot‑specific Certificates of Analysis.
Technical support, documentation and sample requests
Provision of protocols, product inserts, MSDS files, performance notes and publication references; options for requesting samples and technical application support.
Sample preparation and direct‑PCR workflow support
Supply of sample lysis buffers, protease reagents and validated direct‑PCR protocols for a range of clinical, environmental and research sample types, with guidance on inhibitor mitigation and compatibility with inhibitor‑resistant master mixes.
Lyophilized/dry kit formulation for field assays
Development support for glycerol‑free enzyme formulations and master mixes compatible with lyophilization to produce dry kit formats for point‑of‑care or ambient‑stable assays.
Expertise Areas
- qPCR and one‑step RT‑qPCR reagent formulation
- Isothermal amplification chemistry and LAMP kit development
- Recombinant enzyme production and enzyme formulation (polymerases, reverse transcriptases, inhibitors)
- Direct/crude‑sample lysis and direct PCR workflows
Key Technologies
- PCR / qPCR
- One‑step reverse transcription (RT‑qPCR)
- Isothermal amplification (LAMP, RAM, WGA, RPA)
- Hot‑start chemistry via small‑molecule inhibition