Resonance Raman spectroscopy for evaluating mitochondrial function
Inventors
Smith, Lois • Cakir, Bertan D. • Romfh, John Padraic • Chen, Peili • Vakhshoori, Daryoosh
Assignees
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Publication Number
US-12298247-B2
Publication Date
2025-05-13
Expiration Date
Abstract
Apparatuses and methods for using Raman Resonance Spectroscopy to evaluate metabolic and oxygenation status of the eye are disclosed herein. In some embodiments, metabolic mapping of the eye may be performed by aligning a Raman spectrum and a recorded spatial image of the eye.
Core Innovation
The invention provides a device that includes a Raman spectrometer arranged to collect inelastic light and a Raman probe in communication with the Raman spectrometer. The Raman probe is arranged to transmit light to a portion of a subject and collect the inelastic light back to the spectrometer. A first imager is arranged to capture a spatial image of the portion of the subject.
The device is configured to generate a Raman spectrum from the collected inelastic light. Using the Raman spectrum, the device is configured to calculate an oxidized ratio, a reduced ratio, and a total quantity of a first molecule. The calculated oxidized ratio, reduced ratio, and total quantity are derived from Raman spectroscopy measurements associated with the subject portion.
The described system supports spatial/structural correlation by generating Raman information for the subject portion while capturing a spatial image. The additional imaging and alignment enables associating changes with the Raman spectrum, including associating histological changes with the Raman spectrum. The disclosed use contexts relate to assessing retinal metabolic and oxygenation/mitochondrial function using oxidized and reduced mitochondrial states and ratios derived from the Raman spectrum, including 3RMR.
Claims Coverage
The partial content identifies one independent claim (clm-00001). Across this independent claim, the key inventive features include Raman spectroscopy hardware for collecting inelastic light from a subject portion, generation of a Raman spectrum, calculation of oxidized ratio, reduced ratio, and total quantity of a first molecule from the Raman spectrum, together with spatial imaging of the same portion.
Raman spectrometer collecting inelastic light with Raman probe transmission and collection
A Raman spectrometer arranged to collect inelastic light; a Raman probe in communication with the Raman spectrometer, the Raman probe arranged to transmit light to a portion of a subject and collect the inelastic light back to the spectrometer.
Raman spectrum generation and calculation from Raman spectrum
The device is configured to generate a Raman spectrum from the collected inelastic light, and wherein the device is configured to calculate an oxidized ratio, a reduced ratio, and a total quantity of a first molecule using the Raman spectrum.
Spatial imaging of the subject portion
A first imager arranged to capture a spatial image of the portion of the subject.
Overall claim coverage centers on a combined Raman spectroscopy device and spatial imaging arrangement for a subject portion, where a Raman spectrum is generated from collected inelastic light and used to calculate an oxidized ratio, a reduced ratio, and a total quantity of a first molecule.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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