Electrochemical assay device and related methods

Inventors

Harding, Ian

Assignees

Agamatrix Inc

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Publication Number

US-11175256-B2

Patent

Publication Date

2021-11-16

Expiration Date


Abstract

An electrochemical test device is provided having a base layer with a first electrode thereon and a top layer with a second electrode thereon. The two electrodes are separated by a spacer layer having an opening therein, such that a sample-receiving space is defined with one electrode on the top surface, the other electrodes on the bottom surface and side walls formed from edges of the opening in the spacer. Reagents for performing the electrochemical reaction are deposited on one of the electrodes and on the side walls of the sample-receiving space.

Core Innovation

The invention relates to an electrochemical test device that defines a sample-receiving space between a first surface and a second surface using a spacer layer having an opening. The device includes a first substrate with a first electrode and a second substrate with a second electrode disposed thereon, with side walls formed from edges of the opening in the spacer.

The core of the invention is the form and placement of the redox active reagent. The reagent is disposed in a dried layer as a coating that covers at least a portion of the first or second electrode and also covers at least a portion of the side walls within the opening. The dried layer extends as a coating contiguously from the covered electrode and along the side walls.

The invention further provides a method of making the electrochemical test device by forming the spacer layer with an opening on the first substrate, introducing a liquid reagent comprising a redox active material into the opening, and drying the liquid reagent to form the dried reagent layer. The introducing step is performed such that, upon drying, the dried reagent layer is formed contiguously covering at least a portion of the first electrode and at least a portion of the side walls, followed by placing the second substrate aligned to face the first and create the sample-receiving space.

Claims Coverage

The partial content provides two independent claims: one for an electrochemical test device and one for a method of making the device. Each independent claim is centered on a contiguously dried redox-active reagent layer that covers both an electrode and the spacer opening side walls.

Contiguously coated dried redox-active reagent on electrode and side walls

The electrochemical test device includes a spacer with an opening defining a sample-receiving space with side walls formed from edges of the opening, first and second substrates carrying first and second electrodes, and a reagent comprising a redox active material oxidized at the first electrode and reduced at the second electrode when used; the reagent is disposed in a dried layer as a coating at least covering a portion of the first or second electrode and at least a portion of the side walls, wherein the layer extends contiguously as a coating from the covered electrode and along the side walls.

Drying liquid reagent introduced into the spacer opening to form a contiguously covering dried layer

The method forms a first substrate with a first electrode, forms a spacer layer with an opening defining side walls and exposing the first electrode, introduces a liquid reagent comprising a redox active material into the opening, and dries the liquid reagent to form a dried reagent layer; the liquid reagent is introduced so that upon drying the dried reagent layer is formed contiguously covering at least a portion of the first electrode and at least a portion of the side walls, and then places a second substrate having a second electrode aligned to face the first to form the sample-receiving space.

Overall claim coverage in the provided content is focused on two inventive aspects: arranging a dried redox-active reagent as a contiguously extending coating from an electrode onto the spacer opening side walls, and manufacturing the device by introducing a liquid redox reagent into the spacer opening and drying it so the dried coating forms that same contiguous electrode-to-side-wall coverage.

Stated Advantages

Not explicitly described in patent.

Documented Applications

Not explicitly described in patent.

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